De Belder D, Faccone D, Tijet N, Melano R G, Rapoport M, Petroni A, Lucero C, Pasteran F, Corso A, Gomez S A
Servicio Antimicrobianos (National Reference Laboratory in Antimicrobial Resistance - NRLAR), Instituto Nacional de Enfermedades Infecciosas-ANLIS "Dr. Carlos G. Malbrán", Ave. Vélez Sarsfield 563, C1282AFF Buenos Aires, Argentina; Research Career, CONICET (Consejo Nacional de Investigaciones Científicas y Tecnológicas), Argentina.
Public Health Ontario, 661 University Avenue, Toronto M5G 1M1, ON, Canada.
Infect Genet Evol. 2017 Oct;54:374-378. doi: 10.1016/j.meegid.2017.07.019. Epub 2017 Jul 21.
All VIM-producing Enterobacteriaceae (six Enterobacter cloacae) submitted to the Argentinian Reference Laboratory in Antimicrobial Resistance in the period 2008-13 were characterized. The isolates were referred from 6 nosocomial institutions located in 5 different cities across the country. All isolates showed carbapenem disk diffusion inhibition zones ≤22mm and synergism between a carbapenem disk and EDTA/SMA. The six isolates were PCR positive for bla. Imipenem MICs were ≤1 to 8μg/ml. Typing by PFGE and MLST distinguished six pulsotypes and sequence types with bla located on novel class 1 integron arrays: ECL-1: ST182, In883; ECL-2, ST90, In885; ECL-3, ST88, In346 with bla; ECL-4, ST184, In900; ECL-5, ST749-new, In900; ECL-6, ST91 and uncharacterized In. Only ECL-2 was able to transfer bla to E. coli J53 by biparental conjugation. bla was located in plasmids of 53-82Kb and in the chromosome (ECL-1 and ECL-5). The diversity of clones, class 1 integrons, plasmids and location of bla, reveals the plasticity of the genetic elements described and highlights the importance of surveillance programs as tools to identify the transmission of these highly resistant metallo-β-lactamase-producing Enterobacteriaceae.
对2008年至2013年期间提交给阿根廷抗菌药物耐药性参考实验室的所有产VIM的肠杆菌科细菌（6株阴沟肠杆菌）进行了特征分析。这些分离株来自该国5个不同城市的6家医院机构。所有分离株的碳青霉烯纸片扩散抑菌圈≤22mm，且碳青霉烯纸片与EDTA/SMA之间存在协同作用。这6株分离株bla的PCR检测均为阳性。亚胺培南的最低抑菌浓度为≤1至8μg/ml。通过脉冲场凝胶电泳（PFGE）和多位点序列分型（MLST）区分出6种脉冲型和序列型，bla位于新型1类整合子阵列上：ECL-1：ST182，In883；ECL-2，ST90，In885；ECL-3，ST88，In346携带bla；ECL-4，ST184，In900；ECL-5，ST749-new，In900；ECL-6，ST91和未鉴定的In。只有ECL-2能够通过双亲接合将bla转移到大肠杆菌J53。bla位于53-82Kb的质粒和染色体中（ECL-1和ECL-5）。克隆、1类整合子、质粒的多样性以及bla的位置，揭示了所描述的遗传元件的可塑性，并突出了监测计划作为识别这些产高耐药金属β-内酰胺酶肠杆菌科细菌传播工具的重要性。
