Emerg Infect Dis. 2018 Jun;24(6):1010-1019. doi: 10.3201/eid2406.171648.
We performed whole-genome sequencing on 170 clinical carbapenemase-producing Enterobacter spp. isolates collected globally during 2008-2014. The most common carbapenemase was VIM, followed by New Delhi metallo-β-lactamase (NDM), Klebsiella pneumoniae carbapenemase, oxacillin 48, and IMP. The isolates were of predominantly 2 species (E. xiangfangensis and E. hormaechei subsp. steigerwaltii) and 4 global clones (sequence type [ST] 114, ST93, ST90, and ST78) with different clades within ST114 and ST90. Particular genetic structures surrounding carbapenemase genes were circulating locally in various institutions within the same or between different STs in Greece, Guatemala, Italy, Spain, Serbia, and Vietnam. We found a common NDM genetic structure (NDM-GE-U.S.), previously described on pNDM-U.S. from Klebsiella pneumoniae ATCC BAA-214, in 14 different clones obtained from 6 countries spanning 4 continents. Our study highlights the importance of surveillance programs using whole-genome sequencing in providing insight into the molecular epidemiology of carbapenemase-producing Enterobacter spp.
我们对 2008 年至 2014 年间全球收集的 170 株临床产碳青霉烯酶肠杆菌属分离株进行了全基因组测序。最常见的碳青霉烯酶是 VIM，其次是新德里金属β-内酰胺酶（NDM）、肺炎克雷伯菌碳青霉烯酶、耐苯唑西林 48 型和 IMP。这些分离株主要来自 2 个种（阴沟肠杆菌和豪氏厄尔布赖特肠杆菌亚种）和 4 个全球克隆（序列类型[ST]114、ST93、ST90 和 ST78），其中 ST114 和 ST90 内有不同的进化枝。在希腊、危地马拉、意大利、西班牙、塞尔维亚和越南的不同机构中，围绕碳青霉烯酶基因的特定遗传结构在同一 ST 或不同 ST 之间局部循环。我们发现了一种常见的 NDM 遗传结构（NDM-GE-US），之前在肺炎克雷伯菌 ATCC BAA-214 上的 pNDM-US 中描述过，在来自 6 个国家的 14 个不同克隆中都有发现，这些国家分布在 4 个大洲。我们的研究强调了使用全基因组测序进行监测计划的重要性，这为了解产碳青霉烯酶肠杆菌属的分子流行病学提供了深入的认识。
