Zhang Wei-Guo, Yin Xiu-Cai, Liu Xiao-Fang, Meng Ke-Wei, Tang Kun, Huang Fei-Long, Xu Gang, Gao Jie
Department of Hepatobiliary and Pancreatic Surgery, Yantai Yuhuangding Hospital Affiliated to Qingdao University, Yantai, P.R. China
Department of Hepatobiliary Surgery, People's Hospital of Rongcheng, Weihai, P.R. China.
Anticancer Res. 2017 Aug;37(8):4425-4431. doi: 10.21873/anticanres.11837.
BACKGROUND/AIM: Puerarin possesses a battery of therapeutic values in diverse disorders, including pro-apoptotic actions in multiple cancers. Herein, we investigated the effects of puerarin on hepatocellular carcinoma (HCC) in vitro.
MTT and flow cytometry were carried out to evaluate the viability and apoptosis of SMMC-7721 HCC cells in the presence of different concentrations of puerarin. Moreover, expression levels, as well as phosphorylation status of several canonical components in mitogen-activated protein kinase (MAPK) pathways, including extracellular signal-regulated kinase 1/2 (ERK1/2), c- Jun N-terminal kinase (JNK), p38, were measured by reverse transcription and quantitative real-time polymerase chain reaction (RT-PCR) and western blot analysis at indicated time intervals.
Puerarin inhibited proliferation of SMMC-7721 cells and promoted their apoptosis in a dose- and time-dependent fashion (p<0.05). Both the expression and phosphorylation levels of MAPK proteins were dramatically increased on puerarin treatment.
Puerarin could be employed as a potential anti-carcinogen that exhibits pro-apoptotic effects on HCC cells, in a dose- and time-dependent manner, with emphasis on MAPK pathways whose initiation may contribute to this process.
背景/目的：葛根素在多种疾病中具有一系列治疗价值，包括在多种癌症中具有促凋亡作用。在此，我们研究了葛根素在体外对肝癌（HCC）的影响。
采用MTT法和流式细胞术评估不同浓度葛根素作用下SMMC - 7721肝癌细胞的活力和凋亡情况。此外，在指定时间间隔，通过逆转录和定量实时聚合酶链反应（RT-PCR）以及蛋白质印迹分析，检测丝裂原活化蛋白激酶（MAPK）通路中几个典型成分的表达水平以及磷酸化状态，这些成分包括细胞外信号调节激酶1/2（ERK1/2）、c-Jun氨基末端激酶（JNK）、p38。
葛根素以剂量和时间依赖性方式抑制SMMC - 7721细胞的增殖并促进其凋亡（p<0.05）。葛根素处理后，MAPK蛋白的表达和磷酸化水平均显著增加。
葛根素可作为一种潜在的抗癌物质，以剂量和时间依赖性方式对肝癌细胞发挥促凋亡作用，尤其涉及可能在此过程中起作用的MAPK通路。
