Wei Zhaohui, Xu Chao, Wang Juan, Lu Fengxia, Bie Xiaomei, Lu Zhaoxin
College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu, China.
PeerJ. 2017 Jul 19;5:e3601. doi: 10.7717/peerj.3601. eCollection 2017.
This paper is the first public report that can produce both actinomycin D and holomycin. The actinomycete strain NJ-4 isolated from the soil of Nanjing Agricultural University was identified as . This strain was found for the first time to produce two antimicrobial compounds that were identified as actinomycin D and holomycin. GS medium, CS medium and GSS medium were used for the production experiments. All three media supported the production of actinomycin D, while holomycin was detected only in GS medium and was undetectable by HPLC in the CS and GSS media. The antimicrobial activity against , , , , and was tested using the agar well diffusion method. Actinomycin D exhibited strong antagonistic activities against all the indicator strains. Holomycin exhibited strong antagonistic activities against , and and had antifungal activity against and but had no antifungal activity against . The cell viability was determined using an MTT assay. Holomycin exhibited cytotoxic activity against A549 lung cancer cells, BGC823 gastric cancer cells and HepG2 hepatocellular carcinoma cells. The yield of actinomycin D from NJ-4 was 960 mg/l. NJ-4 exhibits a distinct capability and has the industrial potential to produce considerable yields of actinomycin D under unoptimized conditions.
本文是首篇关于能同时产生放线菌素D和全霉素的公开报道。从南京农业大学土壤中分离出的放线菌菌株NJ - 4被鉴定为……该菌株首次被发现能产生两种抗菌化合物，分别鉴定为放线菌素D和全霉素。使用GS培养基、CS培养基和GSS培养基进行生产实验。所有这三种培养基都支持放线菌素D的产生，而全霉素仅在GS培养基中被检测到，在CS和GSS培养基中通过高效液相色谱法无法检测到。使用琼脂扩散法测试了对……、……、……、……、……和……的抗菌活性。放线菌素D对所有指示菌株均表现出强烈的拮抗活性。全霉素对……、……和……表现出强烈的拮抗活性，对……和……具有抗真菌活性，但对……没有抗真菌活性。使用MTT法测定细胞活力。全霉素对A549肺癌细胞、BGC823胃癌细胞和HepG2肝癌细胞表现出细胞毒性活性。NJ - 4产生放线菌素D的产量为960毫克/升。NJ - 4表现出独特的能力，并且在未优化的条件下具有工业潜力，能够大量生产放线菌素D。
