Department of Interventional Ultrasound, Chinese PLA General Hospital, 28 Fuxing Road, Beijing 100853, China.
Nanoscale. 2017 Aug 10;9(31):11068-11076. doi: 10.1039/c7nr02955a.
Microwave ablation (MWA) is a promising minimally invasive therapy that has been widely used to treat hepatocellular carcinoma (HCC). However, the efficiency of MWA in treating HCC is evidently limited by the incomplete ablation of large tumors and tumors in high-risk locations. Here, we report the value of using liposomes packed with sodium chloride (NaCl-LPs) as effective thermo-seeds for MWA of HCC. The prepared liposomes exhibited excellent heat conversion ability by showing a more rapid temperature increase than free NaCl medium, blank liposomes or water under microwave irradiation. The high efficiency of this new microwave sensitization strategy was fully demonstrated in vitro in subcutaneous and orthotopic tumors. The results showed that MWA combined with NaCl-LPs clearly enhanced the ablation efficiency, leading to apparent tumor inhibition and low recurrence. What's more, we verified the susceptibility of NaCl-LPs on orthotopic tumors. Based on the unique properties of NaCl-LPs, sublethal MWA was used to mimic the transitional zone, and large-scale necrosis was observed in tumors combined with NaCl-LPs. In addition, HE staining and blood hematology analysis revealed no noticeable toxicity of NaCl-LPs in vivo, which confirmed that NaCl-LPs possessed good biocompatibility.
The effective nanoparticles could play a valuable role in enhancing the thermo-sensitizing effect of MWA for achieving better therapeutic efficacy.
微波消融（MWA）是一种有前途的微创治疗方法，已广泛用于治疗肝细胞癌（HCC）。然而，MWA 治疗 HCC 的效率显然受到大肿瘤和高危部位肿瘤不完全消融的限制。在这里，我们报告了使用装有氯化钠的脂质体（NaCl-LPs）作为 HCC 的 MWA 的有效热种子的价值。所制备的脂质体在微波辐射下显示出比游离 NaCl 介质、空白脂质体或水更快的升温速度，表现出优异的热转换能力。这种新的微波敏化策略的高效率在皮下和原位肿瘤的体外得到了充分证明。结果表明，MWA 联合 NaCl-LPs 明显提高了消融效率，导致明显的肿瘤抑制和低复发。更重要的是，我们验证了 NaCl-LPs 对原位肿瘤的敏感性。基于 NaCl-LPs 的独特性质，采用亚致死性 MWA 模拟过渡区，与 NaCl-LPs 联合观察到肿瘤内的大规模坏死。此外，HE 染色和血液血液学分析显示 NaCl-LPs 在体内没有明显的毒性，这证实了 NaCl-LPs 具有良好的生物相容性。
有效的纳米颗粒可以在增强 MWA 的热敏化作用方面发挥重要作用，以实现更好的治疗效果。
