Lu Liming, Zeng Jingchun
The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou, China.
The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China.
PLoS One. 2017 Jul 25;12(7):e0181532. doi: 10.1371/journal.pone.0181532. eCollection 2017.
Genetic alterations in K-ras and p53 are thought to be critical in pancreatic cancer development and progression. However, K-ras and p53 expression in pancreatic adenocarcinoma have not been systematically examined in The Cancer Genome Atlas (TCGA) Data Portal. Information regarding K-ras and p53 alterations, mRNA expression data, and protein/protein phosphorylation abundance was retrieved from The Cancer Genome Atlas (TCGA) databases, and analyses were performed by the cBioPortal for Cancer Genomics. The mutual exclusivity analysis showed that events in K-ras and p53 were likely to co-occur in pancreatic adenocarcinoma (Log odds ratio = 1.599, P = 0.006). The graphical summary of the mutations showed that there were hotspots for protein activation. In the network analysis, no solid association between K-ras and p53 was observed in pancreatic adenocarcinoma. In the survival analysis, neither K-ras nor p53 were associated with both survival events. As in the data mining study in the TCGA databases, our study provides a new perspective to understand the genetic features of K-ras and p53 in pancreatic adenocarcinoma.
K-ras和p53基因的改变被认为在胰腺癌的发生和发展中起着关键作用。然而，癌症基因组图谱（TCGA）数据门户尚未对胰腺腺癌中K-ras和p53的表达进行系统研究。从癌症基因组图谱（TCGA）数据库中检索了有关K-ras和p53改变、mRNA表达数据以及蛋白质/蛋白质磷酸化丰度的信息，并通过癌症基因组学cBioPortal进行了分析。互斥性分析表明，K-ras和p53事件在胰腺腺癌中可能同时发生（对数优势比=1.599，P=0.006）。突变的图形总结显示存在蛋白质激活热点。在网络分析中，未观察到胰腺腺癌中K-ras和p53之间有可靠的关联。在生存分析中，K-ras和p53均与生存事件无关。与TCGA数据库中的数据挖掘研究一样，我们的研究为理解胰腺腺癌中K-ras和p53的遗传特征提供了新的视角。
