Rajan Binoy, Patel Deepti M, Kitani Yoichiro, Viswanath Kiron, Brinchmann Monica F
Faculty of Bioscience and Aquaculture, Nord University, 8049 Bodø, Norway.
Faculty of Bioscience and Aquaculture, Nord University, 8049 Bodø, Norway.
Fish Shellfish Immunol. 2017 Sep;68:452-457. doi: 10.1016/j.fsi.2017.07.039. Epub 2017 Jul 22.
This study presents the first report of purification of natterin-like protein (Nlp) in a non-venomous fish. The peptide identities of purified cod Nlp were confirmed through LC-MSMS and matched to a cod expressed sequence tag (EST). A partial cod nlp nucleotide sequence was amplified and sequenced based on this EST. Multiple sequence alignment of cod Nlp showed considerable homology with other teleost Nlps and the presence of an N-terminal jacalin-like lectin domain coupled with a C-terminal toxin domain. nlp expression was higher in skin, head kidney, liver and spleen than in other tissues studied. Hemaggluttination of horse red blood cells by Nlp was calcium dependent and inhibited by mannose. A Vibrio anguillarum bath challenge however, did not alter the expression of cod nlp transcripts in the skin and gills. Further functional characterization is required to establish the significance of this unique protein in Atlantic cod and other teleosts.
本研究首次报道了在一种无毒鱼类中纯化出类纳特林蛋白（Nlp）。通过液相色谱-串联质谱（LC-MSMS）确认了纯化的鳕鱼Nlp的肽段身份，并与鳕鱼表达序列标签（EST）进行了匹配。基于该EST扩增并测序了鳕鱼nlp的部分核苷酸序列。鳕鱼Nlp的多序列比对显示与其他硬骨鱼Nlps具有相当高的同源性，并且存在一个N端的jacalin样凝集素结构域以及一个C端的毒素结构域。nlp在皮肤、头肾、肝脏和脾脏中的表达高于所研究的其他组织。Nlp对马红细胞的血凝作用依赖于钙，并受到甘露糖的抑制。然而，鳗弧菌浸浴攻击并未改变皮肤和鳃中鳕鱼nlp转录本的表达。需要进一步的功能表征来确定这种独特蛋白质在大西洋鳕鱼和其他硬骨鱼中的意义。
