Aquatic Animal Health Research Group, Faculty of Biosciences and Aquaculture, University of Nordland, 8049 Bodø, Norway.
Fish Shellfish Immunol. 2011 Aug;31(2):224-31. doi: 10.1016/j.fsi.2011.05.006. Epub 2011 May 18.
The skin mucosal proteome of Atlantic cod (Gadus morhua) was mapped using a 2D PAGE, LC-MS/MS coupled approach. Mucosal proteins from naive fish were identified primarily by similarity searches across various cod EST databases. The identified proteins were clustered into 8 groups based on gene ontology classification for biological process. Most of the proteins identified from the gel are hitherto unreported for cod. Galectin-1, mannan binding lectin (MBL), serpins, cystatin B, cyclophilin A, FK-506 binding protein, proteasome subunits (alpha-3 and -7), ubiquitin, and g-type lysozyme are considered immune competent molecules. Five of the aforementioned proteins were cloned and their tissue distribution was analysed by RT-PCR.
使用 2D PAGE、LC-MS/MS 偶联方法绘制了大西洋鳕鱼（Gadus morhua）的皮肤黏膜蛋白质组图谱。通过在各种鳕鱼 EST 数据库中进行相似性搜索，鉴定了未致敏鱼类的黏膜蛋白。根据基因本体论对生物学过程的分类，鉴定出的蛋白质被分为 8 组。从凝胶中鉴定出的大多数蛋白质以前尚未在鳕鱼中报道过。半乳糖凝集素-1、甘露糖结合凝集素 (MBL)、丝氨酸蛋白酶抑制剂、半胱氨酸蛋白酶抑制剂 B、亲环蛋白 A、FK-506 结合蛋白、蛋白酶体亚基 (α-3 和 -7)、泛素和 g 型溶菌酶被认为是具有免疫功能的分子。上述 5 种蛋白质被克隆，并通过 RT-PCR 分析其组织分布。
