Carson Richard H, Lewis Charlotte R, Erickson Mercede N, Zagieboylo Anna P, Naylor Bradley C, Li Kelvin W, Farnsworth Paul B, Price John C
Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT 86402.
Independent Researcher Brigham Young University, Provo, UT 86402.
J Lipid Res. 2017 Sep;58(9):1884-1892. doi: 10.1194/jlr.M078170. Epub 2017 Jul 25.
Compartmentalization of metabolism into specific regions of the cell, tissue, and organ is critical to life for all organisms. Mass spectrometric imaging techniques have been valuable in identifying and quantifying concentrations of metabolites in specific locations of cells and tissues, but a true understanding of metabolism requires measurement of metabolite flux on a spatially resolved basis. Here, we utilize desorption ESI-MS (DESI-MS) to measure lipid turnover in the brains of mice. We show that anatomically distinct regions of the brain have distinct lipid turnover rates. These turnover measurements, in conjunction with relative concentration, will enable calculation of regiospecific synthesis rates for individual lipid species in vivo. Monitoring spatially dependent changes in metabolism has the potential to significantly facilitate research in many areas, such as brain development, cancer, and neurodegeneration.
将新陈代谢分隔到细胞、组织和器官的特定区域对所有生物体的生命至关重要。质谱成像技术在识别和量化细胞及组织特定位置的代谢物浓度方面很有价值，但要真正理解新陈代谢，需要在空间分辨的基础上测量代谢物流。在这里，我们利用解吸电喷雾电离质谱（DESI-MS）来测量小鼠大脑中的脂质周转率。我们表明，大脑中解剖学上不同的区域具有不同的脂质周转率。这些周转率测量结果与相对浓度相结合，将能够计算体内单个脂质种类的区域特异性合成率。监测新陈代谢中空间依赖性的变化有可能显著促进许多领域的研究，如大脑发育、癌症和神经退行性变。
