Li Wenjing, Yi Peishan, Zhu Zhiwen, Zhang Xianliang, Li Wei, Ou Guangshuo
Tsinghua-Peking Center for Life Sciences, School of Life Sciences and MOE Key Laboratory for Protein Science, Tsinghua University, Beijing, China.
Tsinghua-Peking Center for Life Sciences, School of Life Sciences and MOE Key Laboratory for Protein Science, Tsinghua University, Beijing, China
EMBO J. 2017 Sep 1;36(17):2553-2566. doi: 10.15252/embj.201796883. Epub 2017 Jul 25.
Neuronal cilia that are formed at the dendritic endings of sensory neurons are essential for sensory perception. However, it remains unclear how the centriole-derived basal body is positioned to form a template for cilium formation. Using fluorescence time-lapse microscopy, we show that the centriole translocates from the cell body to the dendrite tip in the sensory neurons. The centriolar protein SAS-5 interacts with the dynein light-chain LC8 and conditional mutations of cytoplasmic dynein-1 block centriole translocation and ciliogenesis. The components of the central tube are essential for the biogenesis of centrioles, which later drive ciliogenesis in the dendrite; however, the centriole loses these components at the late stage of centriole translocation and subsequently recruits transition zone and intraflagellar transport proteins. Together, our results provide a comprehensive model of ciliogenesis in sensory neurons and reveal the importance of the dynein-dependent centriole translocation in this process.
在感觉神经元树突末端形成的神经元纤毛对于感觉感知至关重要。然而，尚不清楚源自中心粒的基体如何定位以形成纤毛形成的模板。利用荧光延时显微镜，我们发现中心粒在感觉神经元中从细胞体转移到树突尖端。中心粒蛋白SAS-5与动力蛋白轻链LC8相互作用，细胞质动力蛋白-1的条件性突变会阻断中心粒转移和纤毛发生。中心管的成分对于中心粒的生物发生至关重要，中心粒随后驱动树突中的纤毛发生；然而，中心粒在中心粒转移后期会失去这些成分，随后招募过渡区和鞭毛内运输蛋白。总之，我们的结果提供了感觉神经元中纤毛发生的全面模型，并揭示了动力蛋白依赖性中心粒转移在此过程中的重要性。
