Chourabi Kalthoum, Campoy Susana, Rodriguez Jesus A, Kloula Salma, Landoulsi Ahmed, Chatti Abdelwaheb
Laboratory of Wastewater Treatment and Valorization, Water Research and Technology Centre, Technopole of Borj-Cédria, BP 273, Soliman, 8020, Tunisia.
Department of Genetics and Microbiology, Autonomous University of Barcelona, 08290, Barcelona, Spain.
Curr Microbiol. 2017 Nov;74(11):1261-1269. doi: 10.1007/s00284-017-1311-1. Epub 2017 Jul 25.
Water UV disinfection remains extremely important, particularly in developing countries where drinking and reclaimed crop irrigation water may spread devastating infectious diseases. Enteric bacterial pathogens, among which Shigella, are possible contaminants of drinking and bathing water and foods. To study the effect of UV light on Shigella, four strains were exposed to different doses in a laboratory-made irradiation device, given that the ultraviolet radiation degree of inactivation is directly related to the UV dose applied to water. Our results showed that the UV-C rays are effective against all the tested Shigella strains. However, UV-C doses appeared as determinant factors for Shigella eradication. On the other hand, Shigella-survived strains changed their outer membrane protein profiles, secreted proteins, and lipopolysaccharides. Also, as shown by electron microscopy transmission, morphological alterations were manifested by an internal cytoplasm disorganized and membrane envelope breaks. Taken together, the focus of interest of our study is to know the adaptive mechanism of UV-C resistance of Shigella strains.
水的紫外线消毒仍然极为重要，尤其是在发展中国家，那里的饮用水和再生作物灌溉用水可能传播毁灭性的传染病。肠道细菌病原体，其中包括志贺氏菌，可能是饮用水、洗浴用水和食物的污染物。为了研究紫外线对志贺氏菌的影响，在实验室自制的辐照装置中，对四株志贺氏菌菌株进行了不同剂量的照射，因为紫外线的失活程度与施加于水中的紫外线剂量直接相关。我们的结果表明，紫外线C射线对所有测试的志贺氏菌菌株均有效。然而，紫外线C剂量似乎是根除志贺氏菌的决定性因素。另一方面，存活下来的志贺氏菌菌株改变了它们的外膜蛋白谱、分泌蛋白和脂多糖。此外，如透射电子显微镜所示，形态学改变表现为内部细胞质紊乱和膜包膜破裂。综上所述，我们研究的关注点在于了解志贺氏菌菌株对紫外线C抗性的适应性机制。
