Laboratoire de Traitement des Eaux Usées, Centre des Recherches et des Technologies des Eaux CERTE, Technopole Borj Cédria, P.O. Box 273, 8020 Soliman, Tunisia.
Curr Microbiol. 2013 Jul;67(1):112-7. doi: 10.1007/s00284-013-0342-5. Epub 2013 Mar 6.
The changes in lipid composition enable the micro-organisms to maintain membrane functions in the face of environmental fluctuations. The relationship between membrane fatty acid composition and UV-C stress was determined for mid-exponential phase and stationary phase Pseudomonas aeruginosa. The total lipids were obtained by dichloromethane/methanol (3:1) and were quantified by GC. The TLC analysis of phospholipids showed the presence of three major fractions phosphatidylethanolamine, phosphatidylglycerol, and cardiolipin. Significant modifications, as manifested by an increase of UFA, were obtained. Interestingly, this microorganism showed a remarkable capacity for recovery from the stressful effects of UV-C.
脂质组成的变化使微生物能够在面对环境波动时维持膜功能。本研究确定了中对数期和稳定期铜绿假单胞菌的膜脂肪酸组成与 UV-C 应激之间的关系。采用二氯甲烷/甲醇（3:1）提取总脂质并用 GC 定量。磷脂的 TLC 分析表明存在三个主要部分：磷脂酰乙醇胺、磷脂酰甘油和心磷脂。结果表明，不饱和脂肪酸的增加表明发生了显著的变化。有趣的是，这种微生物表现出从 UV-C 应激的影响中恢复的显著能力。
