Hansson E
Life Sci. 1986 Jul 21;39(3):269-77. doi: 10.1016/0024-3205(86)90541-2.
A co-cultivation system was developed with neuron-containing (neuron-specific enolase (NSE) positive) primary cultures from the substantia nigra of 15 to 17-day old embryonic rats which were grown 1 mm apart from astroglial-enriched (glial fibrillary acidic protein (GFAp) positive) primary cultures from the striatum of neonatal rats. The astroglial cells went through a morphological differentiation with extension of processes after co-cultivation with the immunohistochemically-identified neuronal cells. The astroglial-enriched striatum cultures showed a higher active uptake of 3H-L-glutamate after co-cultivation for one week, compared to control cultures from striatum. Vmax (nmol X mg protein-1 X min-1 X was 58.4 +/- 8.3 after co-cultivation and 37.2 +/- 6.3 for control cultures. The glutamine synthetase (GS) activity was slightly increased after co-cultivation. The validity and specificity of the results were ensured. The data suggest that astroglial cells in a primary culture are influenced by co-cultivation with fetal neuron containing cultures resulting in morphological differentiation, and increases in 3H-L-glutamate uptake and GS activity.
构建了一种共培养系统，将来自15至17日龄胚胎大鼠黑质的含神经元（神经元特异性烯醇化酶（NSE）阳性）原代培养物与来自新生大鼠纹状体的富含星形胶质细胞（胶质纤维酸性蛋白（GFAp）阳性）原代培养物相距1毫米培养。与免疫组化鉴定的神经元细胞共培养后，星形胶质细胞经历了形态分化，出现了突起延伸。与来自纹状体的对照培养物相比，富含星形胶质细胞的纹状体培养物在共培养一周后对3H-L-谷氨酸的主动摄取更高。共培养后的Vmax（nmol×mg蛋白-1×min-1）为58.4±8.3，对照培养物为37.2±6.3。共培养后谷氨酰胺合成酶（GS）活性略有增加。结果的有效性和特异性得到了保证。数据表明，原代培养中的星形胶质细胞受到与含胎儿神经元培养物共培养的影响，导致形态分化以及3H-L-谷氨酸摄取和GS活性增加。
