Hansson E
Brain Res. 1986 Feb 26;366(1-2):159-68. doi: 10.1016/0006-8993(86)91291-6.
A technique is described for a two-cell co-cultivation system which permits in vitro evaluation of neuron-glia interactions. Primary astroglial enriched cultures from newborn rat cerebral hemispheres, striatum or cerebral cortex, grown for 3 days, were co-cultivated with primary neuron-containing cultures from 15- to 17-day rat embryo cerebral hemispheres, substantia nigra or brainstem, respectively, grown for 10 days on polylysine-coated surfaces. The neuronal cells were identified morphologically and immunohistochemically by antibodies to neuron-specific enolase. The two cultures were grown together for 7 days, separated by a U-formed 1 mm glass-rod. The results show that neurons exert a morphogenetic effect on astroglial cells in the form of extension of cell processes. The co-culture system allows investigation of potent local humoral interactions between astroglial cells and neurons.
本文描述了一种用于双细胞共培养系统的技术，该技术可在体外评估神经元与神经胶质细胞的相互作用。将新生大鼠大脑半球、纹状体或大脑皮质来源的原代富含星形胶质细胞的培养物培养3天，分别与15至17天大鼠胚胎大脑半球、黑质或脑干来源的含原代神经元的培养物在聚赖氨酸包被的表面培养10天，然后将二者共同培养。通过针对神经元特异性烯醇化酶的抗体，从形态学和免疫组织化学方面对神经元细胞进行鉴定。两种培养物共同培养7天，中间用一根1毫米U形玻璃棒隔开。结果表明，神经元以细胞突起延伸的形式对星形胶质细胞发挥形态发生作用。该共培养系统能够研究星形胶质细胞与神经元之间有效的局部体液相互作用。
