Aoun Meriem, Kolmer James A, Rouse Matthew N, Chao Shiaoman, Bulbula Worku Denbel, Elias Elias M, Acevedo Maricelis
First author: Department of Plant Pathology, North Dakota State University, Fargo 58108; second and third authors: United States Department of Agriculture-Agricultural Research Service (USDA-ARS), Cereal Disease Laboratory, and Department of Plant Pathology, University of Minnesota, St. Paul 55108; fourth author: USDA-ARS Genotyping Laboratory, Biosciences Research Laboratory, Fargo, ND, 58102; fifth author: Ethiopian Institute of Agricultural Research, P.O. Box 32, Debre Zeit, Ethiopia; sixth author: Department of Plant Sciences, North Dakota State University; and seventh author: International Programs, College of Agriculture and Life Sciences, Cornell University, Ithaca, NY.
Phytopathology. 2017 Dec;107(12):1496-1506. doi: 10.1094/PHYTO-12-16-0444-R. Epub 2017 Sep 29.
Leaf rust, caused by Puccinia triticina, and stem rust, caused by P. graminis f. sp. tritici, are important diseases of durum wheat. This study determined the inheritance and genomic locations of leaf rust resistance (Lr) genes to P. triticina race BBBQJ and stem rust resistance (Sr) genes to P. graminis f. sp. tritici race TTKSK in durum accessions. Eight leaf-rust-resistant genotypes were used to develop biparental populations. Accessions PI 192051 and PI 534304 were also resistant to P. graminis f. sp. tritici race TTKSK. The resulting progenies were phenotyped for leaf rust and stem rust response at seedling stage. The Lr and Sr genes were mapped in five populations using single-nucleotide polymorphisms and bulked segregant analysis. Five leaf-rust-resistant genotypes carried single dominant Lr genes whereas, in the remaining accessions, there was deviation from the expected segregation ratio of a single dominant Lr gene. Seven genotypes carried Lr genes different from those previously characterized in durum. The single dominant Lr genes in PI 209274, PI 244061, PI387263, and PI 313096 were mapped to chromosome arms 6BS, 2BS, 6BL, and 6BS, respectively. The Sr gene in PI 534304 mapped to 6AL and is most likely Sr13, while the Sr gene in PI 192051 could be uncharacterized in durum.
由小麦叶锈菌（Puccinia triticina）引起的叶锈病和由小麦秆锈菌（P. graminis f. sp. tritici）引起的秆锈病是硬粒小麦的重要病害。本研究确定了硬粒小麦种质中对小麦叶锈菌生理小种BBBQJ的叶锈病抗性（Lr）基因以及对小麦秆锈菌生理小种TTKSK的秆锈病抗性（Sr）基因的遗传及基因组定位。利用8个抗叶锈病基因型构建双亲群体。种质PI 192051和PI 534304也对小麦秆锈菌生理小种TTKSK具有抗性。对所得后代在苗期进行叶锈病和秆锈病抗性表型鉴定。利用单核苷酸多态性和混合分组分析法在5个群体中对Lr和Sr基因进行定位。5个抗叶锈病基因型携带单个显性Lr基因，而在其余种质中，单个显性Lr基因的分离比出现偏差。7个基因型携带的Lr基因与先前在硬粒小麦中鉴定的基因不同。PI 209274、PI 244061、PI387263和PI 313096中的单个显性Lr基因分别定位到染色体臂6BS、2BS、6BL和6BS上。PI 534304中的Sr基因定位到6AL，很可能是Sr13，而PI 192051中的Sr基因在硬粒小麦中可能尚未鉴定。
