Ding Zhi-Ming, Huang Chun-Jie, Jiao Xiao-Fei, Wu Di, Huo Li-Jun
Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Education Ministry of China, College of Animal Science and Technology, Huazhong, Agricultural University, Wuhan, 430070, China.
Cytoskeleton (Hoboken). 2017 Oct;74(10):369-378. doi: 10.1002/cm.21388. Epub 2017 Aug 23.
TACC3 regulates spindle organization during mitosis and also regulates centrosome-mediated microtubule nucleation by affecting γ-Tubulin ring complexes. In addition, it interacts with different proteins (such as ch-TOG, clathrin and Aurora-A) to function in mitotic spindle assembly and stability. By forming the TACC3/ch-TOG complex, TACC3 acts as a plus end-tracking protein to promote microtubule elongation. The TACC3/ch-TOG/clathrin complex is formed to stabilize kinetochore fibers by crosslinking adjacent microtubules. Furthermore, the phosphorylation of TACC3 by Aurora-A is important for the formation of TACC3/ch-TOG/clathrin and its recruitment to kinetochore fibers. Recently, the aberrant expression of TACC3 in a variety of human cancers has been linked with mitotic defects. Thus, in this review, we will discuss our current understanding of the biological roles of TACC3 in mitotic spindle organization.
TACC3在有丝分裂过程中调节纺锤体组织，还通过影响γ-微管蛋白环复合物来调节中心体介导的微管成核。此外，它与不同的蛋白质（如ch-TOG、网格蛋白和极光激酶A）相互作用，在有丝分裂纺锤体组装和稳定性方面发挥作用。通过形成TACC3/ch-TOG复合物，TACC3作为一种正端追踪蛋白促进微管延长。TACC3/ch-TOG/网格蛋白复合物的形成是通过交联相邻微管来稳定动粒纤维。此外，极光激酶A对TACC3的磷酸化对于TACC3/ch-TOG/网格蛋白的形成及其募集到动粒纤维至关重要。最近，TACC3在多种人类癌症中的异常表达与有丝分裂缺陷有关。因此，在本综述中，我们将讨论目前对TACC3在有丝分裂纺锤体组织中的生物学作用的理解。
