Mingari M C, Gerosa F, Maggi E, Cozzani R, Pende D, Bottino C, Poggi A, Romagnani S, Moretta L
Ric Clin Lab. 1986 Jan-Mar;16(1):23-8. doi: 10.1007/BF02886720.
Human peripheral blood T cells were cloned under conditions allowing the clonal expansion of virtually all T cells. Clones derived from T4+ or T4- (T8+) subsets were screened for their ability to induce B cell proliferation either in the Staphylococcus aureus Cowan-I (SAC)-driven assay or in the costimulation assay based on the use of anti-mu antibodies. BCGF activity in the SAC- and in the anti-mu-driven system was displayed by 13% and 15% T8+ clones, respectively, while 74% and 79% T4+ clones had BCGF activity in the same assay. SN of clones with BCGF activity were further screened for their susceptibility to the inhibition by CM 269 (anti-IL-2 receptor) monoclonal antibody. All clones were inhibited in the SAC assay, whereas several clones were partially or totally resistant to inhibition in the anti-mu assay. Nine of such non-inhibited clones were further analyzed for their ability to produce gamma-IFN (known to act as a BCGF) and IL-2: six of them were found to produce gamma-IFN, while none produced any detectable IL-2 activity.
人外周血T细胞在几乎能使所有T细胞进行克隆扩增的条件下进行克隆。从T4⁺或T4⁻（T8⁺）亚群衍生的克隆，通过在金黄色葡萄球菌考恩I型（SAC）诱导的试验中或基于使用抗μ抗体的共刺激试验中诱导B细胞增殖的能力进行筛选。在SAC诱导和抗μ诱导系统中，分别有13%和15%的T8⁺克隆显示出BCGF活性，而在相同试验中，74%和79%的T4⁺克隆具有BCGF活性。对具有BCGF活性的克隆的上清液进一步筛选其对CM 269（抗IL - 2受体）单克隆抗体抑制作用的敏感性。在SAC试验中所有克隆均受到抑制，而在抗μ试验中，有几个克隆部分或完全抵抗抑制作用。对9个此类未受抑制的克隆进一步分析其产生γ - 干扰素（已知可作为BCGF起作用）和IL - 2的能力：发现其中6个产生γ - 干扰素，而无一产生任何可检测到的IL - 2活性。
