Pena Hilda Fátima de Jesus, Evangelista Camila Mariellen, Casagrande Renata Assis, Biezus Giovana, Wisser Claudia Salete, Ferian Paulo Eduardo, Moura Anderson Barbosa de, Rolim Veronica Machado, Driemeier David, Oliveira Solange, Alves Bruna Farias, Gennari Solange Maria, Traverso Sandra Davi
Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia - FMVZ, Universidade de São Paulo - USP, São Paulo, SP, Brasil.
Departamento de Medicina Veterinária, Centro de Ciências Agroveterinárias - CAV, Universidade do Estado de Santa Catarina - UDESC, Lages, SC, Brasil.
Rev Bras Parasitol Vet. 2017 Apr-Jun;26(2):177-184. doi: 10.1590/S1984-29612017025.
The objective of the study was to report on a fatal case of feline toxoplasmosis with coinfection with the feline leukemia virus (FeLV). A domestic cat (Felis silvestris catus) presented intense dyspnea and died three days later. In the necropsy, the lungs were firm, without collapse and with many white areas; moderate lymphadenomegaly and splenomegaly were also observed. The histopathological examination showed severe necrotic interstitial bronchopneumonia and mild necrotic hepatitis, associated with intralesional cysts and tachyzoites of Toxoplasma gondii that were positive by anti-T. gondii immunohistochemical (IHC) evaluation. The bone marrow showed chronic myeloid leukemia and the neoplastic cells were positive by anti-FeLV IHC evaluation. DNA extracted from lungs was positive for T. gondii by PCR targeting REP-529. T. gondii was characterized by PCR-RFLP and by the microsatellites technique. ToxoDB-PCR-RFLP #10, i.e. the archetypal type I, was identified. Microsatellite analysis showed that the strain was a variant of type I with two atypical alleles. This was the first time that a T. gondii clonal type I genotype was correlated with a case of acute toxoplasmosis in a host in Brazil.
本研究的目的是报告一例猫弓形虫病合并猫白血病病毒（FeLV）感染的致死病例。一只家猫（Felis silvestris catus）出现严重呼吸困难，三天后死亡。尸检时，肺部坚实，无塌陷，有许多白色区域；还观察到中度淋巴结肿大和脾肿大。组织病理学检查显示严重坏死性间质性支气管肺炎和轻度坏死性肝炎，伴有病灶内囊肿和刚地弓形虫速殖子，抗弓形虫免疫组化（IHC）评估呈阳性。骨髓显示慢性髓性白血病，肿瘤细胞经抗FeLV IHC评估呈阳性。通过靶向REP - 529的PCR检测，从肺中提取的DNA对弓形虫呈阳性。通过PCR - RFLP和微卫星技术对弓形虫进行了鉴定。鉴定出ToxoDB - PCR - RFLP #10，即原型I型。微卫星分析表明该菌株是具有两个非典型等位基因的I型变体。这是巴西首次将刚地弓形虫克隆I型基因型与宿主急性弓形虫病病例相关联。
