Santos Tássia L A Dos, Queiroz Raphael F, Sawaya Alexandra C H F, Lopez Begoña Gimenez-Cassina, Soares Milena B P, Bezerra Daniel P, Rodrigues Ana Carolina B C, Paula Vanderlúcia F DE, Waldschmidt Ana Maria
Departamento de Ciências Biológicas, Universidade Estadual do Sudoeste da Bahia, Rua José Moreira Sobrinho, s/n, Jequiezinho, 45208-091 Jequié, BA, Brazil.
Departamento de Ciências Naturais, Universidade Estadual do Sudoeste da Bahia, Estrada do Bem Querer, Km 04, s/n, 45031-900 Vitória da Conquista, BA, Brazil.
An Acad Bras Cienc. 2017;89(3 Suppl):2247-2259. doi: 10.1590/0001-3765201720160725. Epub 2017 Jul 20.
Geopropolis is a special type of propolis produced by stingless bees. Several pharmacological properties have been described for different types of geopropolis, but there have been no previous studies of the geopropolis from Melipona mondury. In this study, we investigated the antioxidant, antibacterial, and antiproliferative activities of M. mondury geopropolis, and determined its chemical profile. The antioxidant activity was determined using in vitro ABTS·+, ·DPPH, and β-carotene/linoleic acid co-oxidation methods. The antibacterial activity was determined using a microdilution method with Pseudomonas aeruginosa, Staphylococcus aureus, and methicillin-resistant S. aureus. The antiproliferative effect was determined in tumor cell lines using the Alamar Blue assay. The chemical profile was obtained using UHPLC-MS and UHPLC-MS/MS. The butanolic fraction had the highest concentration of phenolic compounds and more potent antioxidant properties in all assays. This fraction also had bacteriostatic and bactericidal effects against all bacterial strains at low concentrations, especially S. aureus. The hexane fraction had the highest antiproliferative potential, with IC50 values ranging from 24.2 to 46.6 µg/mL in HL-60 (human promyelocytic leukemia cell) and K562 (human chronic myelocytic leukemia cell), respectively. Preliminary chemical analysis indicates the presence of terpenes and gallic acid in the geopropolis. Our results indicate the therapeutic potential of geopropolis from M. mondury against inflammatory, oxidative, infectious, and neoplastic diseases.
地蜂胶是一种由无刺蜂生产的特殊类型的蜂胶。针对不同类型的地蜂胶，已有多种药理特性被描述，但此前尚无关于蒙杜里无刺蜂地蜂胶的研究。在本研究中，我们调查了蒙杜里无刺蜂地蜂胶的抗氧化、抗菌和抗增殖活性，并确定了其化学组成。抗氧化活性通过体外ABTS·+、·DPPH和β-胡萝卜素/亚油酸共氧化法测定。抗菌活性通过对铜绿假单胞菌、金黄色葡萄球菌和耐甲氧西林金黄色葡萄球菌采用微量稀释法测定。抗增殖作用在肿瘤细胞系中使用alamar蓝测定法测定。化学组成通过超高效液相色谱-质谱联用仪（UHPLC-MS）和超高效液相色谱-串联质谱联用仪（UHPLC-MS/MS）获得。在所有测定中，丁醇馏分的酚类化合物浓度最高，抗氧化性能更强。该馏分在低浓度下对所有细菌菌株也具有抑菌和杀菌作用，尤其是对金黄色葡萄球菌。己烷馏分具有最高的抗增殖潜力，在HL-60（人早幼粒细胞白血病细胞）和K562（人慢性粒细胞白血病细胞）中的IC50值分别为24.2至46.6μg/mL。初步化学分析表明地蜂胶中存在萜类化合物和没食子酸。我们的结果表明蒙杜里无刺蜂地蜂胶对炎症、氧化、感染和肿瘤疾病具有治疗潜力。
