Universidade de Blumenau, Graduation Program in Chemistry, Rua Antonio da Veiga 140, Campus I, Blumenau, SC, Brazil, 89030001.
Universidade de Blumenau, SC, School of Pharmacy, Rua São Paulo 2171, Campus III, Brazil.
Chem Biodivers. 2023 Aug;20(8):e202300592. doi: 10.1002/cbdv.202300592. Epub 2023 Jul 27.
We aimed to characterize and investigate the antibacterial potential of the native stingless bees geopropolis volatile oils (VO) for the search of potentially new bioactive compounds. Geopropolis samples from Melipona bicolor schencki, M. compressipes manaosensis, M. fasciculata, M. quadrifasciata, M. marginata and M. seminigra merrillae were collected from hives in South Brazil. VO were obtained by hydrodistillation and characterised by gas chromatography coupled to mass spectrometry (GC/MS). Antimicrobial activity was assessed by microplate dilution method. The lowest MIC against cell walled bacteria was 219±0 μg mL from M. quadrifasciata geopropolis VO with Staphylococcus aureus. The M. b. schencki geopropolis VO minimal inhibition concentration (MIC) was 424±0 μg mL against all the mycoplasma strains evaluated. Fractionation resulted in the reduction of 50 % of the MIC value from the original oil. However, its compounds' synergism seems to be essential to this activity. Antibiofilm assays demonstrated 15.25 % eradication activity and 13.20 % inhibition of biofilm formation after 24 h for one subfraction at 2× its MIC as the best results found. This may be one of the essential mechanisms by which geopropolis VOs perform their antimicrobial activity.
我们旨在对本土无刺蜜蜂地胶挥发油（VO）进行特征描述和抗菌潜力研究，以寻找具有潜在新生物活性的化合物。我们从南里奥格兰德州的蜂巢中采集了 M. bicolor schencki、M. compressipes manaosensis、M. fasciculata、M. quadrifasciata、M. marginata 和 M. seminigra merrillae 的地胶样本。VO 通过水蒸馏法获得，并通过气相色谱-质谱联用（GC/MS）进行了特征描述。采用微量稀释法评估了抗菌活性。对地胶 VO 最低 MIC 值为 219±0 μg·mL-1，对金黄色葡萄球菌具有抑制作用。M. b. schencki 地胶 VO 对所有评估的支原体菌株的最小抑制浓度（MIC）为 424±0 μg·mL-1。分级分离导致原始油 MIC 值降低了 50%。然而，其化合物的协同作用似乎对这种活性至关重要。抗菌生物膜试验表明，在 24 小时后，一种亚组分在其 MIC 的 2 倍时，对生物膜的清除率为 15.25%，对生物膜形成的抑制率为 13.20%，这是迄今为止发现的最佳结果。这可能是地胶 VO 发挥其抗菌活性的一个重要机制。
