Tiryaki Volkan Müjdat, Ayres Virginia M, Ahmed Ijaz, Shreiber David I
Department of Computer Engineering, School of Engineering and Architecture, Siirt University, Siirt 56100, Turkey.
Department of Electrical and Computer Engineering, Michigan State University, East Lansing, Michigan 48824.
Cytometry A. 2017 Aug;91(8):794-799. doi: 10.1002/cyto.a.23183. Epub 2017 Jul 26.
A novel quantitative volumetric spreading index (VSI) is defined that depends on the total distance between object voxels and the contact surface plane in three-dimensional (3D) space. The VSI, which ranges from 0 to 1, is rotationally invariant around the z-axis. VSI can be used to quantify the degree of individual cell spreading, which is important for analysis of cell interactions with their environment. The VSIs of astrocytes cultured on a nanofibrillar surface and three different comparative planar surfaces have been calculated from confocal laser scanning microscope z-series images, and the effects of both culture surface and immunoreactivity on the degree of cell spreading were investigated. VSI calculations indicated a statistical correlation between increased reactivity, based on immunolabeling for glial fibrillary acidic protein, and decreased cell spreading. Further results provided a quantitative measure for the increased spreading of quiescent-like and reactive-like astrocytes on planar substrates functionalized with poly-l-lysine. © 2017 International Society for Advancement of Cytometry.
定义了一种新型定量体积扩展指数（VSI），它取决于三维（3D）空间中目标体素与接触表面平面之间的总距离。VSI范围为0到1，围绕z轴旋转不变。VSI可用于量化单个细胞的扩展程度，这对于分析细胞与其环境的相互作用很重要。已从共聚焦激光扫描显微镜z系列图像中计算出在纳米纤维表面和三个不同比较平面上培养的星形胶质细胞的VSI，并研究了培养表面和免疫反应性对细胞扩展程度的影响。VSI计算表明，基于胶质纤维酸性蛋白的免疫标记，反应性增加与细胞扩展减少之间存在统计相关性。进一步的结果为静止样和反应样星形胶质细胞在聚-L-赖氨酸功能化的平面基质上扩展增加提供了定量测量方法。©2017国际细胞计量学促进协会。
