Electronic & Biological Nanostructures Laboratory, Department of Electrical and Computer Engineering, 428 S Shaw Lane, Michigan State University, Engineering Building Room 2120, East Lansing, MI, 48824, USA.
Nanomedicine (Lond). 2015 Mar;10(4):529-45. doi: 10.2217/nnm.14.33. Epub 2014 Jul 2.
To investigate the directive importance of nanophysical properties on the morphological and protein expression responses of dibutyryladenosine cyclic monophosphate (dBcAMP)-treated cerebral cortical astrocytes in vitro.
MATERIALS & METHODS: Elasticity and work of adhesion characterizations of culture surfaces were performed using atomic force microscopy and combined with previous surface roughness and polarity results. The morphological and biochemical differentiation of dBcAMP-treated astrocytes cultured on promising nanofibrillar scaffolds and comparative culture surfaces were investigated by immunocytochemistry, colocalization, super resolution microscopy and atomic force microscopy. The dBcAMP-treated astrocyte responses were further compared with untreated astrocyte responses.
RESULTS & CONCLUSION: Nanofibrillar scaffold properties were shown to reduce immunoreactivity responses while poly-L-lysine-functionalized Aclar® (Ted Pella Inc., CA, USA) properties were shown to induce responses reminiscent of glial scar formation. The comparison study indicated that directive cues may differ in wound-healing versus quiescent situations.
探讨纳米物理特性对二丁酰环腺苷单磷酸（dBcAMP）处理体外大脑皮质星形胶质细胞形态和蛋白表达反应的直接影响。
采用原子力显微镜对培养表面的弹性和附着功进行了表征，并结合先前的表面粗糙度和极性结果进行了分析。通过免疫细胞化学、共定位、超分辨率显微镜和原子力显微镜研究了 dBcAMP 处理的星形胶质细胞在有前途的纳米纤维支架和比较培养表面上的形态和生化分化，并将 dBcAMP 处理的星形胶质细胞反应与未处理的星形胶质细胞反应进行了比较。
研究结果表明，纳米纤维支架的特性降低了免疫反应性，而多聚-L-赖氨酸功能化的 Aclar®（Ted Pella Inc.，CA，美国）特性诱导了类似于神经胶质瘢痕形成的反应。比较研究表明，在创伤愈合与静止状态下，定向信号可能不同。
