Forouzanfar Fatemeh, Hosseini Azar, Amiri Mohammad Sadegh, Rakhshandeh Hassan
Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad, Iran.
Department of Biology, Payame Noor University, Tehran, Iran.
Avicenna J Phytomed. 2017 May-Jun;7(3):214-222.
Sleeplessness is the most common sleep disorder. In this study the hypnotic effect of the hydro-alcoholic extract (HAE) of Karel. and its water fraction (WF), ethyl acetate fraction (EAF) and n-butanol fraction (NBF) were studied in mice.
The test compounds were administered intraperitoneally to mice 30 min before the administration of sodium pentobarbital (30 mg/kg.). Moreover, the influence of flumazenil on the hypnotic effect of the extracts was evaluated. Besides, 30 min after administration of HAE, motor coordination (rota-rod test) was assessed. Additionally, LD50 for HAE was determined and the possible neurotoxicity of the extract was tested in neural PC12 cells.
The HAE and NBF decreased the latency of sleep (p<0.05), and significantly increased the duration of sleep (p<0.05) induced by pentobarbital. These effects of were reversed by flumazenil. HAE did not affect the animals' performance on the rota-rod test. The LD50 value for HAE was found to be 4.8 g/kg. HAE and its fractions did not show neurotoxic effects in cultured PC12 cell line.
The results showed that significantly potentiated pentobarbital hypnosis without toxic effect. Probably, its effects are related to its non-polar constituents.
失眠是最常见的睡眠障碍。在本研究中，对卡雷尔水醇提取物（HAE）及其水相部分（WF）、乙酸乙酯相部分（EAF）和正丁醇相部分（NBF）在小鼠中的催眠作用进行了研究。
在给予戊巴比妥钠（30mg/kg）前30分钟，将受试化合物腹腔注射给小鼠。此外，评估了氟马西尼对提取物催眠作用的影响。此外，在给予HAE 30分钟后，评估运动协调性（转棒试验）。另外，测定了HAE的半数致死量（LD50），并在神经PC12细胞中测试了提取物可能的神经毒性。
HAE和NBF缩短了戊巴比妥诱导的睡眠潜伏期（p<0.05），并显著延长了睡眠持续时间（p<0.05）。氟马西尼可逆转这些作用。HAE不影响动物在转棒试验中的表现。发现HAE的LD50值为4.8g/kg。HAE及其各部分在培养的PC12细胞系中未显示神经毒性作用。
结果表明，其显著增强了戊巴比妥的催眠作用且无毒性作用。其作用可能与其非极性成分有关。
