Forouzanfar Fatemeh, Ghazavi Hamed, Vahedi Mohammad Mahdi, Tarrah Kimia, Yavari Zahra, Hosseini Azar, Aghaee Azita, Rakhshandeh Hassan
Neuroscience Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Department of Neuroscience, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Avicenna J Phytomed. 2020 Jan-Feb;10(1):70-77.
Sleep disorders are among the most common psychiatric and medical conditions. In the present study, the hypnotic effect of was studied in mice
The hydro-alcoholic extract (HAE) of and three fractions of it, namely water fraction (WF), ethyl acetate fraction (EAF), and -hexane fraction (NHF), were intraperitoneally (ip) administrated to mice 30 min before injection of sodium pentobarbital (30 mg/kg, ip). Then, 30 min after administration of HAE, motor coordination (rota-rod test) was evaluated. Besides, LD of HAE was determined and the cytotoxicity of HAE was evaluated in PC12 cells using the MTT assay.
HAE 50-200 mg/kg increased the sleeping time. EAF was the only fraction which could prolong the sleep duration and decrease sleep latency. The LD50 value was 4.8 g/kg. The extract induced no cytotoxic effects in PC12 cell line.
The results suggested that potentiates pentobarbital hypnosis without causing toxic effects. Probably, its effects are mediated by the components present in EAF of this plant.
睡眠障碍是最常见的精神和医学病症之一。在本研究中，对[植物名称未给出]在小鼠中的催眠作用进行了研究。
在腹腔注射戊巴比妥钠（30毫克/千克，腹腔注射）前30分钟，将[植物名称未给出]的水醇提取物（HAE）及其三个馏分，即水馏分（WF）、乙酸乙酯馏分（EAF）和正己烷馏分（NHF）腹腔注射给小鼠。然后，在给予HAE 30分钟后，评估运动协调性（转棒试验）。此外，测定了HAE的半数致死量（LD50），并使用MTT法在PC12细胞中评估了HAE的细胞毒性。
50 - 200毫克/千克的HAE延长了睡眠时间。EAF是唯一能延长睡眠时间并减少睡眠潜伏期的馏分。LD50值为4.8克/千克。该提取物在PC12细胞系中未诱导细胞毒性作用。
结果表明，[植物名称未给出]增强了戊巴比妥的催眠作用且未产生毒性作用。其作用可能由该植物EAF中的成分介导。
