Dabbaghmanesh Mohmmad Hossein, Noorafshan Ali, Talezadeh Pedram, Tanideh Nader, Koohpeyma Farhad, Iraji Aida, Bakhshayeshkaram Marzieh, Montazeri-Najafabady Nima
Department of Endocrinology, Endocrinology and Metabolism Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Department of Anatomy, Histomorphometry and Stereology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Avicenna J Phytomed. 2017 May-Jun;7(3):261-274.
Postmenopausal osteoporosis is characterized by increased fracture risk. However, each approved treatment has specific side effects. Therefore, foods with plant origins have increasingly attracted attention as an alternative treatment. Studies have shown that (EA) has antioxidant properties. The present study aimed to investigate the effects of EA hydroalcoholic extract on ovariectomy-induced osteoporosis in rats using stereological methods.
55 female Sprague-Dawley rats were randomly assigned to control, sham operated (normal saline), ovariectomized (OVX), OVX + EA fruit extract (600 mg/kg BW/day), and OVX + estradiol benzoate (3 mg/kg BW) for 16 weeks. Blood samples were collected to measure calcium, phosphorus, and alkaline phosphatase (ALP) plasma levels. Then, specimens from tibia and fifth lumbar vertebra (L5) bones were prepared and stereological analysis was done.
Ovariectomy significantly decreased the calcium level and increased the ALP level in the OVX group. In spite of improvement in calcium hemostasis in groups treated with estrogen and EA fruit extract (p<0.05), only treatment with estrogen was able to reduce ALP levels. Moreover, treatment with EA fruit extract and estrogen caused a significant increase in the number of osteoblasts in vertebra and tibia compared to the OVX group (p<0.05). Estrogen and EA fruit extract were also able to reduce the number of osteoclasts in tibia of the treated OVX rats (p<0.05).
The results showed that EA extract exerted more effects, markedly, on osteoblastogenesis in the OVX rats. Thus, it could be considered as a potential agent to treat patients with osteoporosis.
绝经后骨质疏松症的特征是骨折风险增加。然而，每种获批的治疗方法都有特定的副作用。因此，植物源性食物作为一种替代治疗方法越来越受到关注。研究表明，（EA）具有抗氧化特性。本研究旨在使用体视学方法研究EA水醇提取物对去卵巢诱导的大鼠骨质疏松症的影响。
将55只雌性Sprague-Dawley大鼠随机分为对照组、假手术组（生理盐水）、去卵巢组（OVX）、OVX + EA果实提取物组（600 mg/kg体重/天）和OVX + 苯甲酸雌二醇组（3 mg/kg体重），持续16周。采集血样以测量血浆钙、磷和碱性磷酸酶（ALP）水平。然后，制备胫骨和第五腰椎（L5）骨的标本并进行体视学分析。
去卵巢显著降低了OVX组的钙水平并提高了ALP水平。尽管雌激素和EA果实提取物治疗组的钙稳态有所改善（p<0.05），但只有雌激素治疗能够降低ALP水平。此外，与OVX组相比，EA果实提取物和雌激素治疗导致椎骨和胫骨中成骨细胞数量显著增加（p<0.05）。雌激素和EA果实提取物还能够减少去卵巢大鼠胫骨中的破骨细胞数量（p<0.05）。
结果表明，EA提取物对去卵巢大鼠的成骨作用更为显著。因此，它可被视为治疗骨质疏松症患者的潜在药物。
