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同时补充罗伊氏乳杆菌和氟化钙纳米粒子对去卵巢诱导骨质疏松症的有益影响。

The beneficial effects of simultaneous supplementation of Lactobacillus reuteri and calcium fluoride nanoparticles on ovariectomy-induced osteoporosis.

机构信息

Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran.

Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

出版信息

BMC Complement Med Ther. 2023 Sep 26;23(1):340. doi: 10.1186/s12906-023-04167-6.

DOI:10.1186/s12906-023-04167-6
PMID:37752485
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10521537/
Abstract

BACKGROUND

The development of new strategies to inhibit and/or treat osteoporosis as a chronic systemic disease is one of the most crucial topics. The present study aimed to investigate the simultaneous effects of calcium fluoride nanoparticles (CaF NPs) and lactobacillus reuteri ATCC PTA 6475 (L. reuteri) against osteoporosis in an ovariectomized rat model (OVX).

METHODS

In this study, 18 matured Wistar female rats were randomly assigned into 6 groups, including control, OVX, sham, OVX + L. reuteri, OVX + CaF NPs, and OVX + L. reuteri + CaF NPs. We used OVX rats to simulate post-menopausal osteoporosis, and the treatments were begun two weeks before OVX and continued for four weeks. All groups' blood samples were collected, and serum biomarkers (estrogen, calcium, vitamin D, and alkaline phosphatase (ALP)) were measured. The tibia and Femur lengths of all groups were measured. Histopathological slides of tibia, kidney, and liver tissues were analyzed using the Hematoxylin and Eosin staining method.

RESULTS

Our results revealed that dietary supplementation of L. reuteri and CaF NPs in low doses for 6 weeks did not show adverse effects in kidney and liver tissues. The tibial and femoral lengths of OVX rats as well as the population of osteoblasts and osteocytes and newly generated osteoid in the tibia remarkably increased in the combination therapy group. Moreover, there was a significant increase in serum estrogen levels and a significant decrease in serum calcium and alkaline phosphatase levels in combination treatment groups compared to the OVX groups not receiving the diet.

CONCLUSIONS

Our results suggest the favorable effects of the simultaneous supplementation of L. reuteri and CaF NP to reduce post-menopausal bone loss.

摘要

背景

开发新的策略来抑制和/或治疗骨质疏松症作为一种慢性系统性疾病是最重要的课题之一。本研究旨在探讨氟化钙纳米粒子（CaF NPs）和罗伊氏乳杆菌 ATCC PTA 6475（L. reuteri）联合应用对去卵巢大鼠模型（OVX）骨质疏松症的影响。

方法

本研究将 18 只成熟 Wistar 雌性大鼠随机分为 6 组，包括对照组、OVX 组、假手术组、OVX+L. reuteri 组、OVX+CaF NPs 组和 OVX+L. reuteri+CaF NPs 组。我们使用 OVX 大鼠模拟绝经后骨质疏松症，治疗从 OVX 前两周开始，持续四周。采集所有组的血液样本，测量血清生物标志物（雌激素、钙、维生素 D 和碱性磷酸酶（ALP））。测量所有组的胫骨和股骨长度。使用苏木精和伊红染色法分析胫骨、肾脏和肝脏组织的组织病理学切片。

结果

我们的结果表明，低剂量的 L. reuteri 和 CaF NPs 饮食补充 6 周对肾脏和肝脏组织没有不良影响。与未接受饮食治疗的 OVX 组相比，OVX 大鼠的胫骨和股骨长度以及胫骨中成骨细胞和骨细胞的数量和新生成的类骨质明显增加。此外，联合治疗组的血清雌激素水平显著升高，血清钙和碱性磷酸酶水平显著降低。

结论

我们的结果表明，同时补充 L. reuteri 和 CaF NP 有利于减少绝经后骨丢失。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8f92/10521537/0ad45eadd815/12906_2023_4167_Fig9_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8f92/10521537/0ad45eadd815/12906_2023_4167_Fig9_HTML.jpg

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