Khodabandeh Zahra, Haghighat Sara, Tanideh Nader, Zare Shahrokh, Farrokhi Farnaz, Karandish Maryam, Iraji Aida
Stem Cells Technology Research Center, Shiraz University of Medical Science, Shiraz, Iran.
School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.
Cell Tissue Bank. 2022 Mar;23(1):143-155. doi: 10.1007/s10561-021-09923-x. Epub 2021 Apr 11.
Dental pulp derived-mesenchymal stem cells (DP-MSCs) is considered a suitable are candidate for tissue engineering techniques and osseous reconstruction. Based on the hypothesis that Hypericum perforatum, Elaeagnus Angustifolia and Psidium guajava extracts can be used in cell-based bone tissue engineering due to meagre cytotoxicity response in the cell culture medium, their effects on the viability and metabolic activity of DP-MSCs were investigated and compared with each extract. DP-MSCs were extracted from human dental pulp, characterized by flow cytometry, and differentiated into Osteogenic and adipogenic lineages which were then cultured in different concentrations of E. Angustifolia, H. perforatum and P. guajava extracts at different time intervals followed by MTT assay evaluation. The dental pulp mesenchymal stem cells were evaluated for their plastic adherence ability, fibroblast-like and spindle morphology. According to flow cytometry data, isolated cells from DP-MSCs expressed MSCs markers. A comparison of herbal extracts' concentrations revealed that 500 μg/ml was toxic to dental pulp stem cells, a guide to the toxic dose for DP-MSCs. The P.guajava bore low toxicity and increased dental pulp stem cell viability in comparison to the other two herbal extracts. The hydro-alcoholic extracts of E. Angustifolia, H. perforatum, and P. guajava were efficient in DP-MSCs viability, and therefore were concluded to be useful in maintaining structural and functional cell viability. It was also concluded that the co-culture of stem cells with herbal elements could stimulate endogenous factors to enhance the proliferation and viability of MSCs.
牙髓来源的间充质干细胞（DP-MSCs）被认为是组织工程技术和骨重建的合适候选者。基于贯叶连翘、沙枣和番石榴提取物在细胞培养基中细胞毒性反应较小，可用于基于细胞的骨组织工程这一假设，研究了它们对DP-MSCs活力和代谢活性的影响，并将每种提取物进行了比较。从人牙髓中提取DP-MSCs，通过流式细胞术进行表征，并分化为成骨和成脂谱系，然后在不同时间间隔用不同浓度的沙枣、贯叶连翘和番石榴提取物进行培养，随后进行MTT分析评估。评估牙髓间充质干细胞的贴壁能力、成纤维细胞样和纺锤形形态。根据流式细胞术数据，从DP-MSCs分离出的细胞表达MSCs标志物。草药提取物浓度的比较显示，500μg/ml对牙髓干细胞有毒，这为DP-MSCs的毒性剂量提供了指导。与其他两种草药提取物相比，番石榴的毒性较低，并提高了牙髓干细胞的活力。沙枣、贯叶连翘和番石榴的水醇提取物对DP-MSCs的活力有促进作用，因此得出结论，它们有助于维持细胞的结构和功能活力。研究还得出结论，干细胞与草药成分的共培养可以刺激内源性因子，增强MSCs的增殖和活力。
