de Goes Kelly C G P, da Silva Josué J, Lovato Gisele M, Iamanaka Beatriz T, Massi Fernanda P, Andrade Diva S
Department of General Biology, Biological Sciences Center, State University of Londrina, Londrina, PR, 86051-990, Brazil.
Agronomic Institute of Paraná - IAPAR, Londrina, PR, 86047-902, Brazil.
Antonie Van Leeuwenhoek. 2017 Dec;110(12):1637-1646. doi: 10.1007/s10482-017-0913-8. Epub 2017 Jul 26.
Fine shale particles and retorted shale are waste products generated during the oil shale retorting process. These by-products are small fragments of mined shale rock, are high in silicon and also contain organic matter, micronutrients, hydrocarbons and other elements. The aims of this study were to isolate and to evaluate fungal diversity present in fine shale particles and retorted shale samples collected at the Schist Industrialization Business Unit (Six)-Petrobras in São Mateus do Sul, State of Paraná, Brazil. Combining morphology and internal transcribed spacer (ITS) sequence, a total of seven fungal genera were identified, including Acidiella, Aspergillus, Cladosporium, Ochroconis, Penicillium, Talaromyces and Trichoderma. Acidiella was the most predominant genus found in the samples of fine shale particles, which are a highly acidic substrate (pH 2.4-3.6), while Talaromyces was the main genus in retorted shale (pH 5.20-6.20). Talaromyces sayulitensis was the species most frequently found in retorted shale, and Acidiella bohemica in fine shale particles. The presence of T. sayulitensis, T. diversus and T. stolli in oil shale is described herein for the first time. In conclusion, we have described for the first time a snapshot of the diversity of filamentous fungi colonizing solid oil shale by-products from the Irati Formation in Brazil.
细页岩颗粒和干馏页岩是油页岩干馏过程中产生的废弃物。这些副产品是开采的页岩岩石的小碎片，硅含量高，还含有有机质、微量营养素、碳氢化合物和其他元素。本研究的目的是分离并评估在巴西巴拉那州南圣马特乌斯的巴西国家石油公司页岩工业化业务部（六号）采集的细页岩颗粒和干馏页岩样品中的真菌多样性。结合形态学和内转录间隔区（ITS）序列，共鉴定出七个真菌属，包括嗜酸菌属、曲霉属、枝孢属、赭曲霉属、青霉属、篮状菌属和木霉属。嗜酸菌属是在细页岩颗粒样品中发现的最主要的属，细页岩颗粒是一种高酸性基质（pH 2.4 - 3.6），而篮状菌属是干馏页岩（pH 5.20 - 6.20）中的主要属。赛尤利篮状菌是在干馏页岩中最常发现的物种，而波希米亚嗜酸菌在细页岩颗粒中最常发现。本文首次描述了油页岩中赛尤利篮状菌、多样篮状菌和斯托利篮状菌的存在。总之，我们首次描述了巴西伊拉蒂组固体油页岩副产品上定殖的丝状真菌多样性的概况。
