Departamento de Micologia Prof. Chaves Batista, CB, Universidade Federal de Pernambuco, Av. Professor Nelson Chaves, s/n, Cidade Universitária, Recife, Pernambuco, CEP: 50670-901, Brazil.
Programa de Pós Graduação em Biociência Animal, Departamento de Morfologia e Fisiologia Animal, Universidade Federal Rural de Pernambuco, Rua Manoel de Medeiros, s/n, Dois Irmãos, Recife, Pernambuco, CEP: 52171-900, Brazil.
World J Microbiol Biotechnol. 2018 Oct 28;34(11):162. doi: 10.1007/s11274-018-2547-z.
This study was conducted to report the richness of endophytic Penicillium and Talaromyces species isolated from Tillandsia catimbauensis, a bromeliad endemic in the Brazilian tropical dry forest (Caatinga), to verify their ability to produce the enzyme L-asparaginase and to partially optimise the production of biomass and L-asparaginase of the best enzyme producer. A total of 184 endophytes were isolated, of which 52 (29%) were identified through morphological and phylogenetic analysis using β-tubulin sequences into nine putative species, four in Penicillium and five in Talaromyces. Talaromyces diversus and T. cf. cecidicola were the most frequent taxa. Among the 20 endophytic isolates selected for L-asparaginase production, 10 had the potential to produce the enzyme (0.50-2.30 U/g), especially T. cf. cecidicola URM 7826 (2.30 U/g) and Penicillium sp. 4 URM 7827 (1.28 U/g). As T. cf. cecidicola URM 7826 exhibited significant ability to produce the enzyme, it was selected for the partial optimisation of biomass and L-asparaginase production. Results of the 2 factorial experimental design showed that the highest dry biomass (0.66 g) was obtained under pH 6.0, inoculum concentration of 1 × 10 and 1% L-proline. However, the inoculum concentration was found to be statistically significant, the pH was marginally significant and the concentration of L-proline was not statistically significant. L-Asparaginase production varied between 0.58 and 1.02 U/g and did not reach the optimal point for enzyme production. This study demonstrates that T. catimbauensis is colonised by different Penicillium and Talaromyces species, which are indicated for enzyme production studies.
本研究旨在报道从巴西热带旱生森林（卡廷加）特有物种卡特兰属 Tillandsia catimbauensis 中分离出的内生青霉属和拟青霉属 Penicillium 和 Talaromyces 物种的丰富度，验证它们产生 L-天冬酰胺酶的能力，并部分优化最佳产酶菌的生物量和 L-天冬酰胺酶的生产。共分离出 184 株内生菌，其中 52 株（29%）通过β-微管蛋白序列的形态学和系统发育分析鉴定为 9 个假定种，4 个在青霉属和 5 个在拟青霉属。多形拟青霉和拟青霉 cf. 拟青霉是最常见的分类群。在 20 株用于 L-天冬酰胺酶生产的内生菌中，有 10 株具有产生该酶的潜力（0.50-2.30 U/g），特别是拟青霉 cf. 拟青霉 URM 7826（2.30 U/g）和青霉属 sp. 4 URM 7827（1.28 U/g）。由于拟青霉 cf. 拟青霉 URM 7826 表现出显著的产生酶的能力，因此选择其用于生物量和 L-天冬酰胺酶生产的部分优化。2 因子实验设计的结果表明，在 pH 值为 6.0、接种浓度为 1×10 和 1% L-脯氨酸时，获得了最高的干生物量（0.66 g）。然而，接种浓度被发现具有统计学意义，pH 值具有边缘统计学意义，而 L-脯氨酸的浓度没有统计学意义。L-天冬酰胺酶的产量在 0.58 和 1.02 U/g 之间变化，没有达到酶生产的最佳点。本研究表明，卡特兰属 Tillandsia catimbauensis 被不同的青霉属和拟青霉属 Penicillium 和 Talaromyces 物种定殖，这些物种适合用于酶生产研究。
