Centro de Excelencia en Investigación Biotecnológica Aplicada al Medio Ambiente, Facultad de Ingeniería y Ciencias, Universidad de La Frontera, Av. Francisco Salazar 01145, P.O. Box 54-D, Temuco, Chile.
Scientific and Technological Bioresource Nucleous (BIOREN-UFRO), Universidad de La Frontera, Temuco, Chile.
Environ Sci Pollut Res Int. 2018 Aug;25(22):21296-21307. doi: 10.1007/s11356-017-9790-y. Epub 2017 Jul 26.
The current study aimed to evaluate the removal of a pesticide mixture composed of the insecticides chlorpyrifos (CP) and diazinon (DZ) from liquid medium, soil and a biobed biomixture by a Streptomyces mixed culture. Liquid medium contaminated with 100 mg L CP plus DZ was inoculated with the Streptomyces mixed culture. Results indicated that microorganisms increased their biomass and that the inoculum was viable. The inoculum was able to remove the pesticide mixture with a removal rate of 0.036 and 0.015 h and a half-life of 19 and 46 h for CP and DZ, respectively. The sterilized soil and biobed biomixture inoculated with the mixed culture showed that Streptomyces was able to colonize the substrates, exhibiting an increase in population determined by quantitative polymerase chain reaction (q-PCR), enzymatic activity dehydrogenase (DHA) and acid phosphatase (APP). In both the soil and biomixture, limited CP removal was observed (6-14%), while DZ exhibited a removal rate of 0.024 and 0.060 day and a half-life of 29 and 11 days, respectively. Removal of the organophosphorus pesticide (OP) mixture composed of CP and DZ from different environmental matrices by Streptomyces spp. is reported here for the first time. The decontamination strategy using a Streptomyces mixed culture could represent a promising alternative to eliminate CP and DZ residues from liquids as well as to eliminate DZ from soil and biobed biomixtures.
本研究旨在评估由混合 Streptomyces 培养物从液体介质、土壤和生物床生物混合物中去除由杀虫剂毒死蜱 (CP) 和二嗪磷 (DZ) 组成的农药混合物。将含有 100 mg/L CP 和 DZ 的污染液体培养基接种到混合 Streptomyces 培养物中。结果表明，微生物增加了其生物量，且接种物具有活力。接种物能够以 0.036 和 0.015 h 的半衰期去除农药混合物，CP 和 DZ 的去除率分别为 0.015 和 0.015 h。用混合培养物接种的灭菌土壤和生物床生物混合物表明，Streptomyces 能够在基质中定植，通过定量聚合酶链反应 (q-PCR)、脱氢酶 (DHA) 和酸性磷酸酶 (APP) 的酶活性来确定种群增加。在土壤和生物混合物中，观察到 CP 的去除有限（6-14%），而 DZ 的去除率分别为 0.024 和 0.060 天，半衰期分别为 29 和 11 天。这是首次报道从不同环境基质中用 Streptomyces spp. 去除 CP 和 DZ 组成的有机磷农药 (OP) 混合物。使用混合 Streptomyces 培养物的去污策略可能是一种有前途的替代方法，可用于从液体中去除 CP 和 DZ 残留，以及从土壤和生物床生物混合物中去除 DZ。
