Gilevich I V, Polyakov I S, Porkhanov V A, Chekhonin V P
Research Institute - S. V. Ochapovsky Regional Clinic Hospital No. 1, Krasnodar, Russia.
N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation, Moscow, Russia.
Bull Exp Biol Med. 2017 Jul;163(3):400-404. doi: 10.1007/s10517-017-3813-z. Epub 2017 Jul 26.
We studied the properties of a tissue-engineered trachea consisting of a polyethylene terephthalate scaffold populated with autologous bone marrow mononuclear cells. The tissue-engineered constructs were obtained before surgery, during the postoperative period, and during autopsy. Cytomorphological analysis during the postoperative period showed the presence of mesenchymal stem cells on the inner surface of the implant on day 3 after surgery and cells of the respiratory epithelium on day 10-14. In autopsy samples, single epithelial cells, endothelial cells, and basal cells were found. Biocompatibility of the tissue-engineered trachea with autologous mononuclear cells of the patient was demonstrated.
我们研究了一种组织工程气管的特性，该气管由填充有自体骨髓单个核细胞的聚对苯二甲酸乙二酯支架构成。组织工程构建体在手术前、术后期间以及尸检时获取。术后期间的细胞形态学分析显示，术后第3天植入物内表面存在间充质干细胞，术后第10 - 14天存在呼吸道上皮细胞。在尸检样本中，发现了单个上皮细胞、内皮细胞和基底细胞。证实了该组织工程气管与患者自体单个核细胞的生物相容性。
