• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

体外用于骨组织工程的生物材料上骨髓单个核细胞的表征

Characterization of bone marrow mononuclear cells on biomaterials for bone tissue engineering in vitro.

作者信息

Henrich Dirk, Verboket René, Schaible Alexander, Kontradowitz Kerstin, Oppermann Elsie, Brune Jan C, Nau Christoph, Meier Simon, Bonig Halvard, Marzi Ingo, Seebach Caroline

机构信息

Department of Trauma, Hand and Reconstructive Surgery, Hospital of the Goethe University, Theodor-Stern-Kai 7, 60590 Frankfurt am Main, Germany.

Department of General and Visceral Surgery, Hospital of the Goethe University, 60590 Frankfurt am Main, Germany.

出版信息

Biomed Res Int. 2015;2015:762407. doi: 10.1155/2015/762407. Epub 2015 Feb 23.

DOI:10.1155/2015/762407
PMID:25802865
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4352750/
Abstract

Bone marrow mononuclear cells (BMCs) are suitable for bone tissue engineering. Comparative data regarding the needs of BMC for the adhesion on biomaterials and biocompatibility to various biomaterials are lacking to a large extent. Therefore, we evaluated whether a surface coating would enhance BMC adhesion and analyze the biocompatibility of three different kinds of biomaterials. BMCs were purified from human bone marrow aspirate samples. Beta tricalcium phosphate (β-TCP, without coating or coated with fibronectin or human plasma), demineralized bone matrix (DBM), and bovine cancellous bone (BS) were assessed. Seeding efficacy on β-TCP was 95% regardless of the surface coating. BMC demonstrated a significantly increased initial adhesion on DBM and β-TCP compared to BS. On day 14, metabolic activity was significantly increased in BMC seeded on DBM in comparison to BMC seeded on BS. Likewise increased VEGF-synthesis was observed on day 2 in BMC seeded on DBM when compared to BMC seeded on BS. The seeding efficacy of BMC on uncoated biomaterials is generally high although there are differences between these biomaterials. Beta-TCP and DBM were similar and both superior to BS, suggesting either as suitable materials for spatial restriction of BMC used for regenerative medicine purposes in vivo.

摘要

骨髓单个核细胞（BMCs）适用于骨组织工程。目前在很大程度上缺乏关于BMC在生物材料上黏附需求以及与各种生物材料生物相容性的比较数据。因此，我们评估了表面涂层是否会增强BMC的黏附，并分析了三种不同生物材料的生物相容性。BMCs从人骨髓抽吸样本中纯化而来。对β-磷酸三钙（β-TCP，未涂层或涂有纤连蛋白或人血浆）、脱矿骨基质（DBM）和牛松质骨（BS）进行了评估。无论表面涂层如何，BMC在β-TCP上的接种效率均为95%。与BS相比，BMC在DBM和β-TCP上的初始黏附显著增加。在第14天，接种在DBM上的BMC的代谢活性相比于接种在BS上的BMC显著增加。同样，与接种在BS上的BMC相比，接种在DBM上的BMC在第2天观察到VEGF合成增加。尽管这些生物材料之间存在差异，但BMC在未涂层生物材料上的接种效率总体较高。β-TCP和DBM相似，且均优于BS，表明二者均可作为用于体内再生医学目的的BMC空间限制的合适材料。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14b7/4352750/aff9fdaa1c8a/BMRI2015-762407.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14b7/4352750/50d63c0a4dfb/BMRI2015-762407.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14b7/4352750/a82bb3b4f0e0/BMRI2015-762407.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14b7/4352750/d2bc407e8143/BMRI2015-762407.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14b7/4352750/aff9fdaa1c8a/BMRI2015-762407.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14b7/4352750/50d63c0a4dfb/BMRI2015-762407.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14b7/4352750/a82bb3b4f0e0/BMRI2015-762407.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14b7/4352750/d2bc407e8143/BMRI2015-762407.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14b7/4352750/aff9fdaa1c8a/BMRI2015-762407.004.jpg

相似文献

1
Characterization of bone marrow mononuclear cells on biomaterials for bone tissue engineering in vitro.体外用于骨组织工程的生物材料上骨髓单个核细胞的表征
Biomed Res Int. 2015;2015:762407. doi: 10.1155/2015/762407. Epub 2015 Feb 23.
2
Comparison of human bone marrow stromal cells seeded on calcium-deficient hydroxyapatite, beta-tricalcium phosphate and demineralized bone matrix.接种于缺钙羟基磷灰石、β-磷酸三钙和脱矿骨基质上的人骨髓基质细胞的比较。
Biomaterials. 2003 Jul;24(15):2593-603. doi: 10.1016/s0142-9612(03)00062-0.
3
Comparison of three different types of scaffolds preseeded with human bone marrow mononuclear cells on the bone healing in a femoral critical size defect model of the athymic rat.三种不同类型支架预先接种人骨髓单核细胞在裸鼠股骨大尺寸缺损模型中对骨愈合的比较。
J Tissue Eng Regen Med. 2018 Mar;12(3):653-666. doi: 10.1002/term.2484. Epub 2017 Oct 2.
4
Development of bioactive porous α-TCP/HAp beads for bone tissue engineering.用于骨组织工程的生物活性多孔 α-TCP/HAp 珠的研制。
J Biomed Mater Res A. 2013 Nov;101(11):3295-300. doi: 10.1002/jbm.a.34517. Epub 2013 Aug 26.
5
In vitro and in vivo evaluation of differentially demineralized cancellous bone scaffolds combined with human bone marrow stromal cells for tissue engineering.用于组织工程的差异脱矿松质骨支架与人骨髓基质细胞联合的体外和体内评价
Biomaterials. 2005 Jun;26(16):3173-85. doi: 10.1016/j.biomaterials.2004.08.020.
6
Biomimetic composite coating on rapid prototyped scaffolds for bone tissue engineering.用于骨组织工程的快速原型支架上的仿生复合涂层。
Acta Biomater. 2011 Feb;7(2):809-20. doi: 10.1016/j.actbio.2010.09.010. Epub 2010 Sep 16.
7
Evaluation of partially demineralized osteoporotic cancellous bone matrix combined with human bone marrow stromal cells for tissue engineering: an in vitro and in vivo study.部分脱矿质骨质疏松性松质骨基质与人骨髓基质细胞联合用于组织工程的评估：一项体内外研究
Calcif Tissue Int. 2008 Sep;83(3):176-85. doi: 10.1007/s00223-008-9159-9. Epub 2008 Aug 15.
8
A composite demineralized bone matrix--self assembling peptide scaffold for enhancing cell and growth factor activity in bone marrow.一种复合脱矿骨基质-自组装肽支架，用于增强骨髓中的细胞和生长因子活性。
Biomaterials. 2014 Jul;35(22):5689-99. doi: 10.1016/j.biomaterials.2014.03.079. Epub 2014 Apr 20.
9
Simultaneous cultivation of human endothelial-like differentiated precursor cells and human marrow stromal cells on beta-tricalcium phosphate.人内皮样分化前体细胞与人骨髓基质细胞在β-磷酸三钙上的同步培养。
Tissue Eng Part C Methods. 2009 Dec;15(4):551-60. doi: 10.1089/ten.TEC.2008.0385.
10
Enhanced osteogenesis of β-tricalcium phosphate reinforced silk fibroin scaffold for bone tissue biofabrication.用于骨组织生物制造的β-磷酸三钙增强丝素蛋白支架的成骨增强作用
Int J Biol Macromol. 2017 Feb;95:14-23. doi: 10.1016/j.ijbiomac.2016.11.002. Epub 2016 Nov 3.

引用本文的文献

1
Osteochondral Regeneration Ability of Uncultured Bone Marrow Mononuclear Cells and Platelet-Rich Fibrin Scaffold.未培养的骨髓单个核细胞与富血小板纤维蛋白支架的骨软骨再生能力
Bioengineering (Basel). 2023 May 31;10(6):661. doi: 10.3390/bioengineering10060661.
2
One Stage Masquelets Technique: Evaluation of Different Forms of Membrane Filling with and without Bone Marrow Mononuclear Cells (BMC) in Large Femoral Bone Defects in Rats.一期 Masquelets 技术：评价不同形式的膜填充在和不在骨髓单个核细胞（BMC）存在下对大鼠大股骨缺损的影响。
Cells. 2023 Apr 30;12(9):1289. doi: 10.3390/cells12091289.
3
Clinical outcomes of autologous platelet-rich plasma and bone marrow mononuclear cells grafting combined with core decompression for Association Research Circulation Osseous II-IIIA stage non-traumatic osteonecrosis of the femoral head.

本文引用的文献

1
Mesenchymal stem cell (MSC) and endothelial progenitor cell (EPC) growth and adhesion in six different bone graft substitutes.间充质干细胞（MSC）和内皮祖细胞（EPC）在六种不同骨移植替代物中的生长与黏附
Eur J Trauma Emerg Surg. 2011 Dec;37(6):635-44. doi: 10.1007/s00068-011-0119-0. Epub 2011 Jun 7.
2
Comparative biomechanical and microstructural analysis of native versus peracetic acid-ethanol treated cancellous bone graft.天然松质骨移植骨与过氧乙酸-乙醇处理的松质骨移植骨的生物力学和微观结构比较分析
Biomed Res Int. 2014;2014:784702. doi: 10.1155/2014/784702. Epub 2014 Feb 11.
3
High calcium bioglass enhances differentiation and survival of endothelial progenitor cells, inducing early vascularization in critical size bone defects.
富血小板血浆和骨髓单个核细胞移植联合核心减压治疗 Association Research Circulation Osseous II-IIIA 期非创伤性股骨头坏死的临床疗效。
Int Orthop. 2023 Sep;47(9):2181-2188. doi: 10.1007/s00264-023-05779-w. Epub 2023 Mar 23.
4
Synthetic materials in craniofacial regenerative medicine: A comprehensive overview.颅面再生医学中的合成材料：全面综述。
Front Bioeng Biotechnol. 2022 Nov 9;10:987195. doi: 10.3389/fbioe.2022.987195. eCollection 2022.
5
Intraoperative Creation of Tissue-Engineered Grafts with Minimally Manipulated Cells: New Concept of Bone Tissue Engineering In Situ.术中利用极少操作的细胞构建组织工程移植物：原位骨组织工程的新概念。
Bioengineering (Basel). 2022 Nov 17;9(11):704. doi: 10.3390/bioengineering9110704.
6
In vitro Evaluation of a 20% Bioglass-Containing 3D printable PLA Composite for Bone Tissue Engineering.用于骨组织工程的含20%生物玻璃的3D可打印聚乳酸复合材料的体外评估
Int J Bioprint. 2022 Aug 17;8(4):602. doi: 10.18063/ijb.v8i4.602. eCollection 2022.
7
3D-Printed PLA-Bioglass Scaffolds with Controllable Calcium Release and MSC Adhesion for Bone Tissue Engineering.用于骨组织工程的具有可控钙释放和间充质干细胞粘附功能的3D打印聚乳酸-生物玻璃支架
Polymers (Basel). 2022 Jun 13;14(12):2389. doi: 10.3390/polym14122389.
8
The Induced Membrane Technique-The Filling Matters: Evaluation of Different Forms of Membrane Filling with and without Bone Marrow Mononuclear Cells (BMC) in Large Femoral Bone Defects in Rats.诱导膜技术——填充物很重要：在大鼠大股骨骨缺损中评估有无骨髓单个核细胞（BMC）时不同形式的膜填充情况。
Biomedicines. 2022 Mar 10;10(3):642. doi: 10.3390/biomedicines10030642.
9
Tissue Engineering with Compact Bone-Derived Cell Spheroids Enables Bone Formation around Transplanted Tooth.细胞微球组织工程技术促进移植牙周围骨形成
Tissue Eng Regen Med. 2022 Apr;19(2):377-387. doi: 10.1007/s13770-021-00423-3. Epub 2022 Feb 4.
10
Fibrous Demineralized Bone Matrix (DBM) Improves Bone Marrow Mononuclear Cell (BMC)-Supported Bone Healing in Large Femoral Bone Defects in Rats.纤维脱矿骨基质（DBM）可改善大鼠大股骨缺损中骨髓单个核细胞（BMC）支持的骨愈合。
Cells. 2021 May 19;10(5):1249. doi: 10.3390/cells10051249.
高钙生物玻璃可增强内皮祖细胞的分化和存活能力，诱导临界尺寸骨缺损的早期血管生成。
PLoS One. 2013 Nov 14;8(11):e79058. doi: 10.1371/journal.pone.0079058. eCollection 2013.
4
Co-transplantation of endothelial progenitor cells and mesenchymal stem cells promote neovascularization and bone regeneration.内皮祖细胞与间充质干细胞共移植促进血管新生和骨再生。
Clin Implant Dent Relat Res. 2015 Apr;17(2):353-9. doi: 10.1111/cid.12104. Epub 2013 Jul 12.
5
Fabrication and characterization of regenerated silk scaffolds reinforced with natural silk fibers for bone tissue engineering.采用天然丝纤维增强的再生丝支架的制备与表征及其在骨组织工程中的应用。
J Biomed Mater Res A. 2013 Aug;101(8):2392-404. doi: 10.1002/jbm.a.34537. Epub 2013 Feb 21.
6
Repair of long-bone pseudoarthrosis with autologous bone marrow mononuclear cells combined with allogenic bone graft.自体骨髓单个核细胞联合同种异体骨移植修复长骨假关节。
Cytotherapy. 2013 May;15(5):571-7. doi: 10.1016/j.jcyt.2013.01.004. Epub 2013 Feb 14.
7
The osteogenic differentiation of human bone marrow MSCs on HUVEC-derived ECM and β-TCP scaffold.人骨髓间充质干细胞在人脐静脉内皮细胞衍生细胞外基质和β-TCP 支架上的成骨分化。
Biomaterials. 2012 Oct;33(29):6998-7007. doi: 10.1016/j.biomaterials.2012.06.061. Epub 2012 Jul 15.
8
Safety of mesenchymal stem cells for clinical application.间充质干细胞用于临床应用的安全性。
Stem Cells Int. 2012;2012:652034. doi: 10.1155/2012/652034. Epub 2012 May 16.
9
IGF signaling contributes to malignant transformation of hematopoietic progenitors by the MLL-AF9 oncoprotein.IGF 信号通路通过 MLL-AF9 癌蛋白促进造血祖细胞的恶性转化。
Exp Hematol. 2012 Sep;40(9):715-723.e6. doi: 10.1016/j.exphem.2012.05.003. Epub 2012 May 18.
10
Endothelial progenitor cells improve directly and indirectly early vascularization of mesenchymal stem cell-driven bone regeneration in a critical bone defect in rats.内皮祖细胞可直接和间接改善大鼠临界骨缺损中骨髓间充质干细胞驱动的骨再生的早期血管化。
Cell Transplant. 2012;21(8):1667-77. doi: 10.3727/096368912X638937. Epub 2012 Apr 10.