大鼠和牛胎盘组织中促肾上腺皮质激素样和阿片样激素的存在。

Presence of corticotropin-like and opiate-like hormones in rat and bovine placental tissues.

作者信息

Hon W K, Ng T B

出版信息

Comp Biochem Physiol C Comp Pharmacol Toxicol. 1986;84(2):277-81. doi: 10.1016/0742-8413(86)90094-0.

Abstract

Acid acetone powder of rat placentas was fractionated on Sephadex G-25 into a void volume peak (R-1) and three retarded peaks (R-2, R-3 and R-4). R-3 contained opiate-like activity and R-4 corticotropin-like activity, suggesting that separate corticotropin-like and opiate-like activities with molecular weight smaller than 5000 were present in rat placentas. Acid acetone powder of bovine placentas contained opiate-like activity which was unretarded on Sephadex G-25. Acid acetone powder of rat brains but not those of lungs, livers or kidneys possessed opiate receptor binding and steroidogenic activities, indicating that the activities in placentas were not due to enzymatically generated artifacts or to peptides contained in blood trapped in the organs.

摘要

将大鼠胎盘的酸丙酮粉末在葡聚糖凝胶G - 25上进行分级分离，得到一个空体积峰（R - 1）和三个滞留峰（R - 2、R - 3和R - 4）。R - 3含有阿片样活性，R - 4含有促肾上腺皮质激素样活性，这表明大鼠胎盘中存在分子量小于5000的单独的促肾上腺皮质激素样和阿片样活性。牛胎盘的酸丙酮粉末含有阿片样活性，在葡聚糖凝胶G - 25上不被滞留。大鼠脑的酸丙酮粉末具有阿片受体结合和类固醇生成活性，而肺、肝或肾的酸丙酮粉末则没有，这表明胎盘中的活性不是由于酶促产生的假象或器官中滞留血液所含的肽所致。