Lu Qinkang, Zhao Na, Zha Guiping, Wang Huiyun, Tong Qihu, Xin Shuanghua
Ophthalmology Center, Yinzhou Hospital Affiliated to Medical School of Ningbo University , Ningbo, China .
DNA Cell Biol. 2017 Oct;36(10):837-844. doi: 10.1089/dna.2017.3808. Epub 2017 Jul 27.
Long noncoding RNAs (lncRNAs) have been reported to play vital roles in various human cancers. The aim of this study was to explore the critical role of lncRNA HOXA11-AS in uveal melanoma (UM) progression. Briefly, we found that HOXA11-AS is overexpressed in UM tissues and cells; HOXA11-AS could regulate UM cell growth, invasion, and apoptosis. Mechanistically, RNA immunoprecipitation demonstrated that HOXA11-AS could simultaneously interact with enhancer of zeste homolog 2 (EZH2) to suppress its target p21 protein expression. In addition, we demonstrated that HOXA11-AS functioned as a molecular sponge for miR-124, and overexpression of miR-124 attenuated the proliferation and invasion-promoting effect of HOXA11-AS. Collectively, our findings reveal an oncogenic role for HOXA11-AS in UM tumorigenesis.
据报道，长链非编码RNA（lncRNAs）在多种人类癌症中发挥着至关重要的作用。本研究的目的是探讨lncRNA HOXA11-AS在葡萄膜黑色素瘤（UM）进展中的关键作用。简而言之，我们发现HOXA11-AS在UM组织和细胞中过表达；HOXA11-AS可以调节UM细胞的生长、侵袭和凋亡。机制上，RNA免疫沉淀表明HOXA11-AS可以同时与zeste同源物2（EZH2）增强子相互作用，以抑制其靶标p21蛋白的表达。此外，我们证明HOXA11-AS作为miR-124的分子海绵发挥作用，miR-124的过表达减弱了HOXA11-AS的增殖和促侵袭作用。总的来说，我们的研究结果揭示了HOXA11-AS在UM肿瘤发生中的致癌作用。
