Graduate Institute of Oral Biology, School of Dentistry, National Taiwan University, Taipei, Taiwan.
Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University, Taipei, Taiwan; Department of Dentistry, National Taiwan University Hospital, College of Medicine, National Taiwan University, Taipei, Taiwan.
J Formos Med Assoc. 2017 Nov;116(11):837-843. doi: 10.1016/j.jfma.2017.06.011. Epub 2017 Jul 24.
BACKGROUND/PURPOSE: Langerhans cells (LCs) are antigen-presenting cells. This study assessed the LC counts in 80 dentigerous cysts (DCs).
The CD1a-positive LC numbers in the lining epithelia and subepithelial connective tissues were counted at 80 DC sites without inflammation, 33 DC sites with mild/moderate inflammation, and 9 DC sites with severe inflammation from 80 DC specimens.
The mean LC counts in the lining epithelia and subepithelial connective tissues increased significantly from no inflammation (0.5 ± 0.5 and 0.2 ± 0.3 cell/high-power field or HPF, respectively) through mild/moderate inflammation (6.8 ± 1.8 and 2.4 ± 2.0 cells/HPF, respectively) to severe inflammation DC sites (18.9 ± 7.0 and 6.7 ± 5.8 cells/HPF, respectively; all P-values < 0.001). DC sites with inflammation had thicker lining epithelia than those without inflammation. Moreover, the mean LC counts in the lining epithelia and subepithelial connective tissues of DCs were significantly higher in the thicker lining epithelium (>50 μm) group (7.4 ± 6.5 and 2.6 ± 3.4 cells/HPF, respectively) than in the thinner lining epithelium (≦ 50 μm) group (0.5 ± 0.5 and 0.2 ± 0.3 cells/HPF, respectively; both P-values < 0.001).
A significant association of high-grade inflammation and thick lining epithelium with the increased LC number in DCs is found. The finding of few LCs in the lining epithelia of DCs without inflammation indicates the reduced immunosurveillance ability against DC lining epithelial cells in DC patients. It needs further studies to confirm the role of reduced immunosurveillance in the enlargement of the DC.
背景/目的：朗格汉斯细胞（LCs）是抗原呈递细胞。本研究评估了 80 例含牙囊肿（DC）中的 LC 计数。
在 80 例无炎症、33 例轻度/中度炎症和 9 例重度炎症的 DC 标本中，对无炎症的 80 个 DC 部位、轻度/中度炎症的 33 个 DC 部位和重度炎症的 9 个 DC 部位的衬里上皮和上皮下结缔组织中的 CD1a 阳性 LC 数量进行计数。
无炎症组（分别为 0.5 ± 0.5 和 0.2 ± 0.3 个细胞/高倍视野或 HPF）、轻度/中度炎症组（分别为 6.8 ± 1.8 和 2.4 ± 2.0 个细胞/HPF）和重度炎症 DC 部位（分别为 18.9 ± 7.0 和 6.7 ± 5.8 个细胞/HPF）的衬里上皮和上皮下结缔组织中的 LC 计数均显著增加（所有 P 值均 <0.001）。有炎症的 DC 部位的衬里上皮比无炎症的 DC 部位更厚。此外，衬里上皮厚度>50 μm 组的 DC 中衬里上皮和上皮下结缔组织中的 LC 计数（分别为 7.4 ± 6.5 和 2.6 ± 3.4 个细胞/HPF）显著高于衬里上皮厚度≦50 μm 组（分别为 0.5 ± 0.5 和 0.2 ± 0.3 个细胞/HPF；两者均 P 值 <0.001）。
在 DC 中发现高等级炎症和厚衬里上皮与 LC 数量增加之间存在显著相关性。无炎症的 DC 衬里上皮中 LC 数量较少，表明 DC 患者的 DC 衬里上皮细胞的免疫监视能力降低。需要进一步研究来确认免疫监视减少在 DC 增大中的作用。
