Chang Chun-Han, Wu Yang-Che, Wu Yu-Hsueh, Sun Andy, Chen Hsin-Ming, Lin Hung-Pin
Graduate Institute of Oral Biology, School of Dentistry, National Taiwan University, Taipei, Taiwan.
Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University, Taipei, Taiwan.
J Dent Sci. 2017 Sep;12(3):283-290. doi: 10.1016/j.jds.2017.04.001. Epub 2017 May 9.
BACKGROUND/PURPOSE: Langerhans cells (LCs) are antigen-presenting cells. This study mainly evaluated the LC counts in 60 odontogenic keratocysts (OKCs).
The CD1a-positive LC numbers in the lining epithelia and subepithelial connective tissues were counted at 60 OKC sites without inflammation, 39 OKC sites with mild/moderate inflammation, and 13 OKC sites with severe inflammation from 60 OKC specimens.
The mean CD1a-positive LC counts in the lining epithelia and subepithelial connective tissues increased significantly from no inflammation (0.5 ± 0.4 and 0.2 ± 0.3 cell/high-power field or HPF, respectively) through mild/moderate inflammation (5.3 ± 2.5 and 2.5 ± 2.7 cells/HPF, respectively) to severe inflammation OKC sites (12.7 ± 5.6 and 9.3 ± 7.2 cells/HPF, respectively; all P-values < 0.001). OKC sites with inflammation had thicker lining epithelia than those without inflammation. Moreover, the mean CD1a-positive LC counts in the lining epithelia and subepithelial connective tissues of OKCs were significantly higher in the thicker lining epithelium (>100 μm) group (6.8 ± 5.1 and 3.7 ± 4.9 cells/HPF, respectively) than in the thinner lining epithelium (≦100 μm) group (1.0 ± 1.7 and 0.8 ± 2.5 cell/HPF, respectively; both P-values < 0.001).
There is a significant association of inflammation grade with the number of LCs in OKCs. The scarce LCs in the lining epithelia of OKCs without inflammation suggests the loss of immunosurveillance ability against the OKC lining epithelial cells; this can explain why OKCs have aggressive clinical behavior, a great growth potential, and a high recurrence rate.
背景/目的：朗格汉斯细胞（LCs）是抗原呈递细胞。本研究主要评估60例牙源性角化囊肿（OKCs）中的LC计数。
从60例OKC标本中，对60个无炎症的OKC部位、39个有轻/中度炎症的OKC部位和13个有重度炎症的OKC部位的衬里上皮和上皮下结缔组织中的CD1a阳性LC数量进行计数。
衬里上皮和上皮下结缔组织中CD1a阳性LC的平均计数从无炎症（分别为0.5±0.4和0.2±0.3个细胞/高倍视野或HPF）到轻/中度炎症（分别为5.3±2.5和2.5±2.7个细胞/HPF）再到重度炎症的OKC部位（分别为12.7±5.6和9.3±7.2个细胞/HPF）显著增加（所有P值<0.001）。有炎症的OKC部位的衬里上皮比无炎症的更厚。此外，OKC衬里上皮和上皮下结缔组织中CD1a阳性LC的平均计数在较厚衬里上皮（>100μm）组（分别为6.8±5.1和3.7±4.9个细胞/HPF）显著高于较薄衬里上皮（≤100μm）组（分别为1.0±1.7和0.8±2.5个细胞/HPF；两个P值<0.001）。
炎症分级与OKC中LC的数量存在显著关联。无炎症的OKC衬里上皮中LC稀少表明对OKC衬里上皮细胞的免疫监视能力丧失；这可以解释为什么OKC具有侵袭性的临床行为、巨大的生长潜力和高复发率。
