Collins L E, Clarke R W, Maskell R
Lancet. 1986 Aug 30;2(8505):479-81. doi: 10.1016/s0140-6736(86)90356-9.
In a 2-month prospective study of streptococci isolated from urine specimens in the laboratory, 242 strains of catalase-negative gram-positive cocci or coccobacilli were isolated in substantial numbers from 11,725 specimens. These comprised 10% of the important isolates. Species identification of all isolates was undertaken. 74 (30%) of the isolates were of species other than Streptococcus faecalis and S agalactiae. 79 (33%) were not detected on cysteine-lactose-electrolyte-deficient agar after overnight incubation in a carbon dioxide incubator. 20 of the 24 isolates of coccobacilli were Gardnerella vaginalis. Many of the isolates of fastidious species were accompanied by pyuria. An isolation protocol practicable in busy laboratories is proposed.
在实验室对从尿液标本中分离出的链球菌进行的为期2个月的前瞻性研究中,从11725份标本中大量分离出242株过氧化氢酶阴性革兰氏阳性球菌或球杆菌。这些占重要分离株的10%。对所有分离株进行了菌种鉴定。74株(30%)分离株为粪肠球菌和无乳链球菌以外的菌种。79株(33%)在二氧化碳培养箱中过夜培养后,在半胱氨酸-乳糖-电解质缺乏琼脂上未检测到。24株球杆菌分离株中有20株为阴道加德纳菌。许多苛养菌分离株伴有脓尿。提出了一种在繁忙实验室可行的分离方案。