Buu-Hoï A, Le Bouguénec C, Horaud T
Centre Hospitalo-Universitaire, Broussais-Hôtel Dieu, Paris, France.
Antimicrob Agents Chemother. 1989 Apr;33(4):529-34. doi: 10.1128/AAC.33.4.529.
Resistance to at least one of the following antibiotics was found in eight wild-type strains of Aerococcus viridans: erythromycin (six strains), tetracycline and minocycline (five strains), chloramphenicol (one strain), and high levels of streptomycin (one strain). None of the strains transferred any of their antibiotic resistance markers into streptococcal, enterococcal, or A. viridans recipients by conjugation. By DNA-DNA hybridization experiments, the ermB gene of transposon Tn917, of Enterococcus faecalis origin, was detected in five of the six strains resistant to erythromycin and was localized for one strain on the chromosome and for four strains on nonconjugative small (4.7- to 4.9-kilobase) plasmids. The tetM gene of the conjugative transposon Tn916, of E. faecalis origin, was localized on the chromosome of four of the five strains resistant to tetracycline and minocycline; in three of these strains a structure similar to that of Tn916 was found. Homology to the tetO gene of pUA466, of Campylobacter jejuni origin, was detected on the chromosome of the fifth strain. No sequence homology was detected in any strain with probes corresponding to the tetL gene of group B Streptococcus origin, to the ermA gene of the transposon Tn554 of Staphylococcus aureus origin, or to the cat genes of either pC194 or pC221 of S. aureus origin.
红霉素（六株）、四环素和米诺环素（五株）、氯霉素（一株）以及高水平链霉素（一株）。通过接合试验，没有一株菌将其任何抗生素耐药性标记转移到链球菌、肠球菌或绿色气球菌受体中。通过DNA-DNA杂交实验，在六株对红霉素耐药的菌株中的五株中检测到了源自粪肠球菌的转座子Tn917的ermB基因，其中一株定位于染色体上，四株定位于非接合性小（4.7至4.9千碱基）质粒上。源自粪肠球菌的接合转座子Tn916的tetM基因定位于五株对四环素和米诺环素耐药的菌株中的四株的染色体上；在其中三株菌株中发现了与Tn916相似的结构。在第五株菌株的染色体上检测到了与空肠弯曲菌来源的pUA466的tetO基因的同源性。在任何菌株中均未检测到与B组链球菌来源的tetL基因探针、金黄色葡萄球菌来源的转座子Tn554的ermA基因探针或金黄色葡萄球菌来源的pC194或pC221的cat基因探针的序列同源性。
