Jokipii A M, Jokipii L
J Clin Microbiol. 1979 Aug;10(2):218-21. doi: 10.1128/jcm.10.2.218-221.1979.
One hundred strains of group B streptococci isolated from human infections were tested for growth on dip-slides available for the culture of urine. All grew on CLED agar, and none grew on MacConkey agar. The colonies were barely or not at all visible to the naked eye after overnight incubation (diameter, around 0.1 mm). The colony size increased eith prolonged incubation, but not if the inoculum density exceeded 10(6)/ml. Differences were found between lots of dip-slides. Poor growth on dip-slides may explain why group B streptococci have received little attention as pathogens of the urinary tract. The dip-slide screening personnel of one laboratory were informed of the experimental findings, and they started the practice of frequent subculture and prolonged incubation. The proportion of group B streptococci in significant bacteriuria increased from 0 to about 2% of positive cultures, whereas there was no conmitant increase of group B streptococci in dip-slides screened in several other laboratories serving as controls.
对从人类感染中分离出的100株B族链球菌进行了检测，观察它们在可用于尿液培养的浸涂玻片上的生长情况。所有菌株都能在CLED琼脂上生长，而在麦康凯琼脂上均不生长。过夜培养后，菌落肉眼几乎不可见或根本不可见（直径约0.1毫米）。随着培养时间延长，菌落大小会增加，但如果接种密度超过10(6)/ml则不会增加。不同批次的浸涂玻片之间存在差异。浸涂玻片上生长不佳可能解释了为什么B族链球菌作为泌尿道病原体很少受到关注。一个实验室的浸涂玻片筛查人员得知了实验结果，他们开始频繁传代培养并延长培养时间。在有意义的菌尿症中，B族链球菌在阳性培养物中的比例从0增加到约2%，而在其他几个作为对照的实验室筛查的浸涂玻片中，B族链球菌并没有相应增加。
