Petermann Julie, Bonnefond Romain, Mermoud Isabelle, Rantoen Dewi, Meynard Laure, Munro Christopher, Lua Linda H L, Hüe Thomas
Équipe ARBOREAL: AgricultuRe BiOdiveRsité Et vALorisation, Laboratoire de Parasitologie, Institut Agronomique néo-Calédonien, BP 73, 98890, Païta, New Caledonia.
Station zootechnique de port Laguerre, Direction Provinciale du Développement Rural, Païta, New Caledonia.
Exp Appl Acarol. 2017 Jul;72(3):303-315. doi: 10.1007/s10493-017-0156-4. Epub 2017 Jul 27.
Cattle tick infestations remain an important burden for farmers in tropical area like in New Caledonia. With the development of acaricide resistance, tick vaccines should be an attractive alternative to control ticks but their efficacy needs to be improved. In this study three adjuvants were studied in an experimental tick vaccine with a Bm86 protein to assess their performance in terms of antibody productions and adverse reactions following vaccinations. The water-in-oil adjuvant ISA 61 VG led to higher antibody titers compared to a water-in-oil-in-water adjuvant ISA 201 VG and an aqueous polymeric adjuvant Montanide Gel 01. Vaccinations with these three adjuvants did not produce severe general reaction but an increase in skin thickness was observed especially with both oil-based emulsions. These results indicated that the water-in-oil adjuvant is the most interesting to use for this vaccine but local adverse reactions remain an issue.
牛蜱感染仍然是新喀里多尼亚等热带地区农民面临的一项重大负担。随着杀螨剂耐药性的发展，蜱疫苗应是控制蜱虫的一种有吸引力的替代方法，但它们的功效需要提高。在本研究中，在一种含有Bm86蛋白的实验性蜱疫苗中研究了三种佐剂，以评估它们在疫苗接种后抗体产生和不良反应方面的表现。与水包油包水佐剂ISA 201 VG和水性聚合物佐剂Montanide Gel 01相比，油包水佐剂ISA 61 VG导致更高的抗体滴度。用这三种佐剂进行疫苗接种未产生严重的全身反应，但观察到皮肤厚度增加，尤其是使用两种油基乳剂时。这些结果表明，油包水佐剂是该疫苗最值得使用的佐剂，但局部不良反应仍然是一个问题。
