Guan Li-Jun, Pei Shi-Xuan, Song Ji-Jian, Zhan Peng-Fei, Han Yi-Nong, Xue Yun, Ding Ke, Zhao Zhan-Qin
Lab of Veterinary Microbiology, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, China.
College of Veterinary Medicine, Jilin University, Changchun, China.
Front Vet Sci. 2022 Jul 26;9:922867. doi: 10.3389/fvets.2022.922867. eCollection 2022.
In this study, we screened adjuvants for an inactivated vaccine against (). Inactivated cells of strain HG-1 were prepared as the antigen in five adjuvanted inactivated vaccines, including a mineral-oil-adjuvanted vaccine (Oli vaccine), aluminum-hydroxide-gel-adjuvanted vaccine (Alh vaccine), ISA201-biphasic-oil-emulsion-adjuvanted vaccine (ISA201 vaccine), GEL02-water-soluble-polymer-adjuvanted vaccine (GEL vaccine), and IMS1313-water-soluble-nanoparticle-adjuvanted vaccine (IMS1313 vaccine). The safety test results of subcutaneous inoculation in mice showed that Oli vaccine had the most severe side effects, with a combined score of 35, followed by the ISA201 vaccine (25 points), Alh vaccine (20 points), GEL vaccine (10 points), and IMS1313 vaccine (10 points). A dose of 1.5LD of strain HG-1 was used to challenge the mice intraperitoneally, 14 days after their second immunization. The protective efficacy of Oli vaccine and Alh vaccine was 100% (8/8), whereas that of the other three adjuvanted vaccines was 88% (7/8). Challenge with 2.5LD of strain HG-1 resulted in a 100% survival rate, demonstrating the 100% protective efficacy of the Oli vaccine, followed by the GEL vaccine (71%, 5/7), IMS1313 vaccine (57%, 4/7), ISA201 vaccine (43%, 3/7), and Alh vaccine (29%, 2/7). Challenge with 4LD of strain HG-1 showed 100% (7/7) protective efficacy of the Oli vaccine and 71% (5/7) protective efficacy of the GEL vaccine, whereas the protective efficacy of other three adjuvanted vaccine was 14% (1/7). The Alh and GEL vaccines were selected for comparative tests in piglets, and both caused minor side effects. A second immunization with these two adjuvanted vaccines conferred 60 and 100% protective efficacy, respectively, after the piglets were challenged via an ear vein with 8LD of strain HG-1. After challenge with 16LD of strain HG-1, the Alh and GEL vaccines showed 40% and 100% protective efficacy, respectively. Our results suggested that GEL is the optimal adjuvant for an inactivated vaccine against .
在本研究中，我们筛选了针对（）的灭活疫苗的佐剂。制备了HG-1株的灭活细胞作为五种佐剂灭活疫苗中的抗原，包括矿物油佐剂疫苗（Oli疫苗）、氢氧化铝凝胶佐剂疫苗（Alh疫苗）、ISA201双相油乳剂佐剂疫苗（ISA201疫苗）、GEL02水溶性聚合物佐剂疫苗（GEL疫苗）和IMS1313水溶性纳米颗粒佐剂疫苗（IMS1313疫苗）。小鼠皮下接种的安全性测试结果表明，Oli疫苗的副作用最严重，综合评分为35分，其次是ISA201疫苗（25分）、Alh疫苗（20分）、GEL疫苗（10分）和IMS1313疫苗（10分）。在第二次免疫后14天，用1.5LD的HG-1株腹腔注射攻击小鼠。Oli疫苗和Alh疫苗的保护效力为100%（8/8），而其他三种佐剂疫苗的保护效力为88%（7/8）。用2.5LD的HG-1株攻击导致100%的存活率，表明Oli疫苗的保护效力为100%，其次是GEL疫苗（71%，5/7）、IMS1313疫苗（57%，4/7）、ISA201疫苗（43%，3/7）和Alh疫苗（29%，2/7）。用4LD的HG-1株攻击显示Oli疫苗的保护效力为100%（7/7），GEL疫苗的保护效力为71%（5/7），而其他三种佐剂疫苗的保护效力为14%（1/7）。选择Alh疫苗和GEL疫苗在仔猪中进行比较试验，两者均引起轻微副作用。在用8LD的HG-1株通过耳静脉攻击仔猪后，用这两种佐剂疫苗进行第二次免疫分别赋予60%和100%的保护效力。在用16LD的HG-1株攻击后，Alh疫苗和GEL疫苗分别显示40%和100%的保护效力。我们的结果表明，GEL是针对（）的灭活疫苗的最佳佐剂。
