Suzuki Koichiro, Shinzawa Naoaki, Ishigaki Keisuke, Nakamura Keiji, Abe Hiroyuki, Fukui-Miyazaki Aya, Ikuta Kazuyoshi, Horiguchi Yasuhiko
Department of Molecular Bacteriology, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamada-oka, Suita, Osaka 565-0871, Japan.
Research Foundation for Microbial Diseases of Osaka University (BIKEN), 3-1 Yamada-oka, Suita, Osaka 565-0871, Japan.
Microbiol Immunol. 2017 Sep;61(9):371-379. doi: 10.1111/1348-0421.12504.
Bordetella pertussis causes whooping cough, a severe and prolonged respiratory disease that results inhas high morbidity and mortality rates, particularly in developing countries. The number incidence of whooping cough cases is increasing in many countries despite high vaccine coverage. Causes for the re-emergence of the disease include the limited duration of protection conferred by the acellular pertussis vaccines (aP)s and pathogenic adaptations that involve antigenic divergence from vaccine strains. Therefore, current vaccines therefore need to be improved. In the present study, we focused on five autotransporters: namely SphB1, BatB, SphB2, Phg, and Vag8, which were previously found to be expressed by B. bronchiseptica during the course of infection in rats and examined their protective efficiencies as vaccine antigens. The passenger domains of these proteins were produced in recombinant forms and used as antigens. An intranasal murine challenge assay showed that immunization with a mixture of SphB1 and Vag8 (SV) significantly reduced bacterial load in the lower respiratory tract and a combination of aP and SV acts synergistically in effects of conferring protection against B. pertussis infection, implying that these antigens have potential as components to for improvinge th the currently available acellular pertussis vaccine.
百日咳博德特氏菌可引发百日咳，这是一种严重且病程迁延的呼吸道疾病，发病率和死亡率都很高，在发展中国家尤为如此。尽管疫苗接种覆盖率很高，但许多国家百日咳病例的发病率仍在上升。该疾病再度出现的原因包括无细胞百日咳疫苗（aP）提供的保护持续时间有限，以及病原体发生适应性变化，导致其抗原与疫苗株出现差异。因此，当前的疫苗需要改进。在本研究中，我们聚焦于五种自转运蛋白，即SphB1、BatB、SphB2、Phg和Vag8，此前发现它们在支气管败血博德特氏菌感染大鼠的过程中表达，我们检测了它们作为疫苗抗原的保护效率。这些蛋白质的乘客结构域以重组形式产生并用作抗原。鼻内小鼠攻毒试验表明，用SphB1和Vag8（SV）的混合物免疫可显著降低下呼吸道的细菌载量，并且aP与SV联合使用在提供针对百日咳博德特氏菌感染的保护作用方面具有协同效应，这意味着这些抗原具有作为改进现有无细胞百日咳疫苗成分的潜力。
