Wang Jun-Bo, Liu Si-Jia, Chen Hong-Ying, Wang Pu
Department of Orthopaedics, West China Hospital, Sichuan University, Chengdu 610041, China.
Center of Rehabilitation Medicine, West China Hospital, Sichuan University, Chengdu 610041, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2017 Jul;48(4):537-542.
To determine the effects of low-magnitude whole body vibration (WBV) on the structure and function of subchondral trabecular bones, cartilage degradation, bone/cartilage turnover, and osteoarthritis (OA) joint function.
Knee osteoarthritis model was established in 96 rabbits through left anterior cruciate ligament transaction (ACLT). The rabbits were randomly divided into six groups: ACLT control group, WBV+ACLT group (five subgroups, each comprising 16 rabbits receiving 5 Hz, 10 Hz, 20 Hz, 30 Hz and 40 Hz WBV, respectively, with 2-4 mm amplitude for 40 min/d and 5 d/week over a period of 8 weeks). Joint function was tested via weight-bearing asymmetry. The microarchitecture of subchondral trabecular bones was examined using vivo micro-computed tomography (micro-CT). Cartilage samples from knee joints were taken for gross morphology and histology examinations. Serum samples were taken to detect cartilage oligomeric matrix protein (COMP), C-terminal telopeptide of type Ⅰ collagen (CTX)-Ⅰ and urine CTX-Ⅱ.
Knee joint pain decreased with 10 Hz (<0.05) and 20 Hz WBV treatment (<0.05) , but increased with 40 Hz treatment (<0.05). The micro-CT results showed that articular cartilage increased first, peaked at 20 Hz, and then decreased (<0.05) . With increased frequency of WBV, the trabecular number, subchondral bone thickness and bone volume fraction increased, serum CTX-Ⅰ decreased, COMP and CTX-Ⅱ increased, especially at 20 Hz (<0.05).
Lower frequency (20 Hz) WBV can improve bone microstructure, increase bone turnover, delay cartilage degeneration and improve limb function of rabbits with OA.
确定低强度全身振动（WBV）对软骨下小梁骨结构和功能、软骨降解、骨/软骨周转以及骨关节炎（OA）关节功能的影响。
通过左前交叉韧带切断术（ACLT）在96只兔中建立膝关节骨关节炎模型。将兔随机分为六组：ACLT对照组、WBV+ACLT组（五个亚组，每组16只兔，分别接受5Hz、10Hz、20Hz、30Hz和40Hz的WBV，振幅为2-4mm，每天40分钟，每周5天，共8周）。通过负重不对称测试关节功能。使用活体微计算机断层扫描（micro-CT）检查软骨下小梁骨的微观结构。采集膝关节软骨样本进行大体形态学和组织学检查。采集血清样本检测软骨寡聚基质蛋白（COMP）、Ⅰ型胶原C末端肽（CTX）-Ⅰ和尿CTX-Ⅱ。
10Hz（<0.05）和20Hz WBV治疗可使膝关节疼痛减轻（<0.05），但40Hz治疗时疼痛增加（<0.05）。micro-CT结果显示，关节软骨先增加，在20Hz时达到峰值，然后下降（<0.05）。随着WBV频率增加，小梁数量、软骨下骨厚度和骨体积分数增加，血清CTX-Ⅰ降低，COMP和CTX-Ⅱ增加，尤其是在20Hz时（<0.05）。
较低频率（20Hz）的WBV可改善OA兔的骨微观结构，增加骨周转，延缓软骨退变并改善肢体功能。
