Murthy S N, Lavy A, Morgantini D S, Chey W Y
Peptides. 1986;7 Suppl 1:229-36. doi: 10.1016/0196-9781(86)90191-9.
We examined the effects of cholinergic, peptidergic and GABAergic agents on secretin secretion from canine duodenal mucosal explants incubated in organ culture media. Carbachol (10(-12) to 10(-4) M), atropine (10(-6) to 10(-4) M), hexamethonium (10(-6) to 10(-4) M), and somatostatin did not alter basal secretion of secretin. Somatostatin (10(-7) to 10(-8) M) inhibited secretin secretion stimulated by pH 4.5. Met, Leu and their D-ala2-analogs inhibited both basal and pH 4.5-stimulated secretin. Naloxone reversed the inhibition caused by met-enkephalin at pH 7.4. GABA (10(-9) to 10(-6) M) stimulated both basal and pH 4.5-stimulated secretin secretion. GABA-stimulated secretin secretion was neuronal in nature, bicuculline sensitive and was mediated via post ganglionic cholinergic neurons. GABA-stimulated secretin secretion was inhibited by both somatostatin and metenkephalin, suggesting that GABA-stimulated secretin secretion may be under the inhibitory control of peptidergic agents as well.
我们研究了胆碱能、肽能和γ-氨基丁酸能药物对在器官培养基中培养的犬十二指肠黏膜外植体分泌促胰液素的影响。卡巴胆碱（10⁻¹²至10⁻⁴M）、阿托品（10⁻⁶至10⁻⁴M）、六甲铵（10⁻⁶至10⁻⁴M）和生长抑素均未改变促胰液素的基础分泌。生长抑素（10⁻⁷至10⁻⁸M）抑制了由pH 4.5刺激的促胰液素分泌。甲硫氨酸脑啡肽、亮氨酸脑啡肽及其D-丙氨酸²类似物抑制了基础分泌以及由pH 4.5刺激的促胰液素分泌。纳洛酮逆转了在pH 7.4时由甲硫氨酸脑啡肽引起的抑制作用。γ-氨基丁酸（10⁻⁹至10⁻⁶M）刺激了基础分泌以及由pH 4.5刺激的促胰液素分泌。γ-氨基丁酸刺激的促胰液素分泌本质上是神经元性的，对荷包牡丹碱敏感，并通过节后胆碱能神经元介导。γ-氨基丁酸刺激的促胰液素分泌受到生长抑素和甲硫氨酸脑啡肽的抑制，这表明γ-氨基丁酸刺激的促胰液素分泌可能也受到肽能药物的抑制性控制。
