Poitras P, Dumont A, Cuber J C, Trudel L
André-Viallet Clinical Research Center, Hôpital St-Luc, University of Montreal, Quebec, Canada.
Peptides. 1993 Mar-Apr;14(2):207-13. doi: 10.1016/0196-9781(93)90031-b.
An in vitro model was developed to study the regulation of motilin release from its endocrine-producing cell. Enzymatically dispersed cells from canine duodenojejunal mucosa were separated by centrifugal counterflow elutriation to enrich motilin content. From the motilin-enriched cell preparation, the release of motilin was determined under stimulation with various agents. Carbachol dose-dependently stimulated the release of motilin from its producing cell. Bombesin, morphine, and erythromycin, recognized stimulants for motilin release in vivo, failed to influence the secretion of motilin in vitro. Serotonin, GIP, CCK, pentagastrin, cisapride, neosynephrine, isoproterenol, or muscimol were also ineffective in the in vitro model. The response to carbachol was abolished by atropine but was not affected by somatostatin, serotonin, secretin, CCK, or GIP. These results suggest that muscarinic receptors are present on the motilin cell membrane and that acetylcholine is a major regulator of motilin release.
建立了一种体外模型，用于研究胃动素从其内分泌产生细胞释放的调节机制。通过离心逆流淘析法分离犬十二指肠空肠黏膜的酶分散细胞，以富集胃动素含量。从富含胃动素的细胞制剂中，测定各种试剂刺激下胃动素的释放情况。卡巴胆碱剂量依赖性地刺激胃动素从其产生细胞中释放。蛙皮素、吗啡和红霉素是体内公认的胃动素释放刺激剂，但在体外未能影响胃动素的分泌。血清素、胃抑肽、胆囊收缩素、五肽胃泌素、西沙必利、去氧肾上腺素、异丙肾上腺素或蝇蕈醇在体外模型中也无效。阿托品可消除对卡巴胆碱的反应，但生长抑素、血清素、促胰液素、胆囊收缩素或胃抑肽对其无影响。这些结果表明，胃动素细胞膜上存在毒蕈碱受体，乙酰胆碱是胃动素释放的主要调节因子。
