基于焦磷酸测序的结核分枝杆菌基因分型新方法——PyroTyping。
PyroTyping, a novel pyrosequencing-based assay for Mycobacterium tuberculosis genotyping.
机构信息
Servei de Microbiologia, Hospital Universitari Germans Trias i Pujol, Institut d'Investigació Germans Trias i Pujol, Universitat Autònoma de Barcelona, Carretera del Canyet s/n, 08916, Badalona, Spain.
CIBER Enfermedades Respiratorias (CIBERES), Instituto de Salud Carlos III, Madrid, 28029, Spain.
出版信息
Sci Rep. 2017 Jul 28;7(1):6777. doi: 10.1038/s41598-017-06760-5.
We developed a novel method, PyroTyping, for discrimination of Mycobacterium tuberculosis isolates combining pyrosequencing and IS6110 polymorphism. A total of 100 isolates were analysed with IS6110-restriction fragment length polymorphism (RFLP), spoligotyping, mycobacterial interspersed repetitive units - variable number tandem repeats (MIRU-VNTR), and PyroTyping. PyroTyping results regarding clustering or discrimination of the isolates were highly concordant with the other typing methods performed. PyroTyping is more rapid than RFLP and presents the same discriminatory power, thus, it may be useful for taking timely decisions for tuberculosis control.
我们开发了一种新的方法，即 PyroTyping，通过焦磷酸测序和 IS6110 多态性来区分结核分枝杆菌分离株。共分析了 100 株分离株的 IS6110 限制性片段长度多态性（RFLP）、 spoligotyping、分枝杆菌插入重复单位 - 可变数串联重复（MIRU-VNTR）和 PyroTyping。PyroTyping 关于分离株聚类或区分的结果与其他进行的分型方法高度一致。PyroTyping 比 RFLP 更快，且具有相同的区分能力，因此，它可能有助于及时做出结核病控制决策。
Zotero 插件