Atia Mohamed A M, Sakr Mahmoud M, Adawy Sami S
Molecular Genetics and Genome Mapping Laboratory (MGGM), Agricultural Genetic Engineering Research Institute (AGERI), ARC, Giza, P.O. Box 12619, Egypt.
Plant Biotechnology Department, National Research Center, Dokki, Giza, Egypt.
Methods Mol Biol. 2017;1638:125-142. doi: 10.1007/978-1-4939-7159-6_12.
Molecular marker technologies which rely on DNA analysis provide powerful tools to assess biodiversity at different levels, i.e., among and within species. A range of different molecular marker techniques have been developed and extensively applied for detecting variability in date palm at the DNA level. Recently, the employment of gene-targeting molecular marker approaches to study biodiversity and genetic variations in many plant species has increased the attention of researchers interested in date palm to carry out phylogenetic studies using these novel marker systems. Molecular markers are good indicators of genetic distances among accessions, because DNA-based markers are neutral in the face of selection. Here we describe the employment of multidisciplinary molecular marker approaches: amplified fragment length polymorphism (AFLP), start codon targeted (SCoT) polymorphism, conserved DNA-derived polymorphism (CDDP), intron-targeted amplified polymorphism (ITAP), simple sequence repeats (SSR), and random amplified polymorphic DNA (RAPD) to assess genetic diversity in date palm.
依赖DNA分析的分子标记技术为评估不同层面的生物多样性提供了强大工具，即在物种间和物种内。一系列不同的分子标记技术已被开发并广泛应用于检测海枣在DNA水平上的变异性。最近，在许多植物物种中使用基因靶向分子标记方法来研究生物多样性和遗传变异，这增加了对海枣感兴趣的研究人员使用这些新型标记系统进行系统发育研究的关注度。分子标记是种质间遗传距离的良好指标，因为基于DNA的标记在面对选择时是中性的。在这里，我们描述了多学科分子标记方法的应用：扩增片段长度多态性（AFLP）、起始密码子靶向（SCoT）多态性、保守DNA衍生多态性（CDDP）、内含子靶向扩增多态性（ITAP）、简单序列重复（SSR）和随机扩增多态性DNA（RAPD），以评估海枣的遗传多样性。
