利用随机扩增多态性DNA（RAPD）和简单重复序列区间（ISSR）对海枣进行遗传多样性分析

Genetic Diversity Analysis of Date Palm Using Random Amplified Polymorphic DNA (RAPD) and Inter-Simple Sequence Repeat (ISSR).

作者信息

Mahatma Mahesh K, Srivashtav Vishal S, Jha Sanjay

机构信息

ICAR-Directorate of Groundnut Research, Junagadh, Gujarat, India.

Department of Plant Molecular Biology and Biotechnology, Navsari Agricultural University, Navsari, Gujarat, India.

出版信息

Methods Mol Biol. 2017;1638:105-112. doi: 10.1007/978-1-4939-7159-6_10.

DOI:10.1007/978-1-4939-7159-6_10

PMID:28755218

Abstract

Random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) are PCR-based molecular techniques used for evaluation and characterization of date palm to find the best genotype and male/female identification at an early stage. Genetic fingerprinting using molecular markers is an important tool for the analysis of genetic diversity and cultivar identification. Here, we present an improved DNA extraction protocol using leaf tissue, based on the standard cetyltrimethyl ammonium bromide (CTAB) protocol, which yields large amounts of high-quality amplifiable DNA. RAPD and ISSR markers reveal sufficient genetic diversity as well as give some unique markers in some genotypes with a maximum number of bands.

摘要

随机扩增多态性DNA（RAPD）和简单重复序列区间（ISSR）是基于聚合酶链反应（PCR）的分子技术，用于枣椰树的评估和特征分析，以便在早期阶段找到最佳基因型并鉴定雌雄株。使用分子标记进行遗传指纹分析是分析遗传多样性和品种鉴定的重要工具。在此，我们基于标准的十六烷基三甲基溴化铵（CTAB）方案，提出了一种使用叶片组织的改进DNA提取方案，该方案可产生大量高质量的可扩增DNA。RAPD和ISSR标记揭示了足够的遗传多样性，并且在一些具有最大条带数的基因型中给出了一些独特的标记。