Al-Faifi Sulieman A, Migdadi Hussein M, Algamdi Salem S, Khan Mohammad Altaf, Al-Obeed Rashid S, Ammar Megahed H, Jakse Jerenj
Plant Production Department, College of Food and Agricultural Sciences, King Saud University, P.O. Box 2460, Riyadh, 11451, Saudi Arabia.
Biotechnical Faculty, Agronomy Department, University of Ljubljana, Ljubljana, Slovenia.
Methods Mol Biol. 2017;1638:283-313. doi: 10.1007/978-1-4939-7159-6_23.
Expressed sequence tags (EST) were generated from a normalized cDNA library of the date palm Sukkari cv. to understand the high-quality and better field performance of this well-known commercial cultivar. A total of 6943 high-quality ESTs were generated, out of them 6671 are submitted to the GenBank dbEST (LIBEST_028537). The generated ESTs were assembled into 6362 unigenes, consisting of 494 (14.4%) contigs and 5868 (84.53%) singletons. The functional annotation shows that the majority of the ESTs are associated with binding (44%), catalytic (40%), transporter (5%), and structural molecular (5%) activities. The blastx results show that 73% of unigenes are significantly similar to known plant genes and 27% are novel. The latter could be of particular interest in date palm genetic studies. Further analysis shows that some ESTs are categorized as stress/defense- and fruit development-related genes. These newly generated ESTs could significantly enhance date palm EST databases in the public domain and are available to scientists and researchers across the globe. This knowledge will facilitate the discovery of candidate genes that govern important developmental and agronomical traits in date palm. It will provide important resources for developing genetic tools, comparative genomics, and genome evolution among date palm cultivars.
为了解著名商业品种枣椰树苏卡里（Sukkari cv.）的高品质和更好的田间表现，从其标准化cDNA文库中生成了表达序列标签（EST）。共生成了6943个高质量EST，其中6671个已提交至GenBank dbEST（LIBEST_028537）。生成的EST被组装成6362个单基因，包括494个（14.4%）重叠群和5868个（84.53%）单拷贝序列。功能注释表明，大多数EST与结合（44%）、催化（40%）、转运（5%）和结构分子（5%）活性相关。blastx结果显示，73%的单基因与已知植物基因显著相似，27%为新基因。后者可能在枣椰树遗传研究中特别受关注。进一步分析表明，一些EST被归类为与胁迫/防御和果实发育相关的基因。这些新生成的EST可显著增强公共领域的枣椰树EST数据库，并可供全球的科学家和研究人员使用。这些知识将有助于发现控制枣椰树重要发育和农艺性状的候选基因。它将为开发遗传工具、比较基因组学以及枣椰树品种间的基因组进化提供重要资源。
