Othmani Ahmed, Bayoudh Chokri, Sellemi Amel, Drira Noureddine
Laboratoire de Culture In Vitro, Centre Régional de Recherches en Agriculture Oasiennes, Degache, Tunisia.
Centre Régional de Recherches en Horticulture et Agriculture Biologique, Chott Mariem, Tunisie.
Methods Mol Biol. 2017;1637:239-249. doi: 10.1007/978-1-4939-7156-5_20.
The temporary immersion system (TIS) is being used with tremendous success for automation of micropropagation of many plant species. TIS usually consists of a culture vessel comprising two compartments, an upper one with the plant material and a lower one with the liquid culture medium and an automated air pump. The latter enables contact between all parts of the explants and the liquid medium by setting overpressure to the lower part of the container. These systems are providing the most satisfactory conditions for date palm regeneration via shoot organogenesis and allow a significant increase of multiplication rate (5.5-fold in comparison with that regenerated on agar-solidified medium) and plant material quality, thereby reducing production cost.
临时浸没系统（TIS）在许多植物物种的微繁殖自动化方面取得了巨大成功。TIS通常由一个培养容器组成，该容器有两个隔层，上部放置植物材料，下部装有液体培养基和一个自动气泵。通过给容器下部施加超压，气泵使外植体的所有部分与液体培养基接触。这些系统为枣椰树通过芽器官发生进行再生提供了最理想的条件，显著提高了繁殖率（与在琼脂固化培养基上再生的相比提高了5.5倍）和植物材料质量，从而降低了生产成本。
